Establishment of an in Vitro Peyers Patch Cell Culture System Correlative to in Vivo Study Using Intestine and Screening of Lactic Acid Bacteria Enhancing Intestinal Immunity
スポンサーリンク
概要
- 論文の詳細を見る
Some lactic acid bacteria (LAB) are known as representative of probiotics. To screen LAB effective to enhance intestinal immunity, in the present study, we developed an accurate and convenient in vitro evaluation system using Peyers patch cells (PP-cells) isolated from the mice intestine. We observed that the amount of immunoglobulin A (IgA) produced by PP-cells co-cultured with LAB was well correlative to that in PP-cells, intestine and feces isolated from live mice after oral administration of LAB [correlation coefficient (r)=0.888, 0.883, and 0.920, respectively]. In addition, using this in vitro system, we suggest that the IgA level of PP-cells co-culturing with plant-derived LAB might be more enhanced than with animal-derived LAB.
- 公益社団法人 日本薬学会の論文
著者
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SUGIYAMA Masanori
Department of Fermentation Technology, Faculty of Engineering, Hiroshima University
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Kumagai Takanori
Department of Internal Medicine, Arita Kyouritsu Hospital, Japan
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NODA Masafumi
Department of Thoracic Surgery, institute of Development, Aging, and Cancer, Tohoku University
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Sugiyama Masanori
Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
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Matoba Yasuyuki
Department of Molecular Microbiology and Biotechnology, Graduate School of Biomedical Sciences, Hiro
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Noda Masafumi
Department Of Artificial System And Engineering Chiba University
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Matoba Yasuyuki
Department Of Molecular Microbiology And Biotechnology Graduate School Of Biomedical Sciences Hirosh
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Jin Hekui
Department of Molecular Microbiology and Biotechnology, Graduate School of Biomedical Sciences, Hiro
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Higashikawa Fumiko
Department of Molecular Nutrition Sciences, Graduate School of Biomedical Sciences, Hiroshima Univer
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Zhao Xingrong
Department of Molecular Microbiology and Biotechnology, Graduate School of Biomedical Sciences, Hiro
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Sugiyama Masanori
Department of Molecular Microbiology and Biotechnology, Graduate School of Biomedical Sciences, Hiroshima University
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Zhao Xingrong
Department of Molecular Microbiology and Biotechnology, Graduate School of Biomedical Sciences, Hiroshima University
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Noda Masafumi
Department of Molecular Nutrition Sciences, Graduate School of Biomedical Sciences, Hiroshima University
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