Identification of Cholesterol Oxidase from Fast-Growing Mycobacterial Strains and Rhodococcus sp.
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概要
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Cholesterol oxidase synthesis ability by various fast-growing mycobacteria (M. fortuitum, M. vaccae, M. phlei, M. smegmatis) and Rhodococcus sp. IM 58 was investigated by thin-layer chromatography and Western blot analysis. In contrast with the case of cholesterol oxidases from Arthrobacter sp. IM 79 and Streptomyces sp., those from the strains tested were localized intracellularly. Western blot experiments revealed high antigenic similarity of those enzymatic proteins to cholesterol oxidase from Schizophyllum commune, and lack of homology to cholesterol oxidase from Streptomyces sp. Differences in the analyzed enzymes were due not only to a single antigenic determinant alteration but also to the significant distinctions on the genetic level as shown by Southern blot hybridization.
- 1995-02-25
著者
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Murooka Yoshikatsu
Depart. Of Bioteclnology Graduate School Of Eng. Osaka Univ.
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Dziadek J
Center Of Microbiology And Virology Polish Acad. Sci. Lodz Pol
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WILMANSKA DANUTA
Department of Industrial Microbiology, Institute of Microbiology and Immunology, University of Lodz
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DZIADEK JAROSLAW
Centre of Microbiology and Virology, Polish Academy of Sciences
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SAJDUDA ANNA
Department of Genetics of Microorganisms, Institute of Microbiology and Immunology, University of Lo
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MILCZAREK KRYSTYNA
Department of Industrial Microbiology, Institute of Microbiology and Immunology, University of Lodz
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JAWORSKI ADAM
Centre of Microbiology and Virology, Polish Academy of Sciences
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Sajduda Anna
Department Of Genetics Of Microorganisms Institute Of Microbiology And Immunology University Of Lodz
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Jaworski Adam
Centre Of Microbiology And Virology Polish Academy Of Sciences:department Of Genetics Of Microorgani
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Wilmanska Danuta
Department Of Industrial Microbiology Institute Of Microbiology And Immunology University Of Lodz
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Milczarek Krystyna
Department Of Industrial Microbiology Institute Of Microbiology And Immunology University Of Lodz
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