PACAP-induced depolarizations in hamster submandibular ganglion neurons

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In this study, we have investigated the effects of pituitary adenylate cyclase-activating polypeptide (PACAP) on m vitro hamster submandibular ganglion neurons using the conventional mtracellular recording technique PACAP (10/μM) induced slow depolarizations in approximately 70% of tested cells PACAP-mduced depolarizations were approximately 10 mV in the peak amplitude, and their durations were approximately 10 mm The slow depolarizations were accompanied by a decrease in membrane conductance (gm) at the initial phase and an increase in gm at the peak phase Membrane input resistance increased by14 8±22% (mean±SE, max ) of the resting value at the initial phase and decreased by 30 8 ± 4 3% (max) at the peak phase Anodal break spikes were elicited at the initial phase during PACAP-mduced depolarization In one neuron, anodal break spikes were elicited at the peak Spikes which followed the anodal break spike were also elicited at 4 Hz in the initial phase dunng the slow depolarizations The decrease in gm was probably produced by an inhibition of calcium conductance and an inhibition of slow Ca^<2+> -activated K^+ channels, while the increase in g_m might have been produced by an activation of nonselective cation channels The slow depolarizations by PACAP might be mediated by a membrane-delimited signal transduction cascade involving G protein in the submandibular ganglion neurons
東京歯科大学の論文