Purification and Properties of Endoxylanase Produced by Bacillus pumilus
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概要
- 論文の詳細を見る
Extracellular endoxylanase (1, 4-β-D-xylan xylanohydrolase [EC 3.2.1.8]) produced by Bacillus pumilus IPO, an isolate from soil in Thailand, was purified to homogeneity. The optimum pH and temperature of the purified xylanase for hydrolysis of larchwood xylan were 6.5 and 40°C. Its molecular weight was estimated to be 24, 000 dalton by SDS-polyacrylamide gel electrophoresis and 20, 000 dalton by equilibrium centrifugation. The number of amino acid residues per molecule was calculated to be 185, including one cysteine. The maximumdegree of hydrolysis of larchwood xylan by the purified xylanase was 25%; xylobiose, xylotriose, xylotetraose and xylopentaose but not xylose were detected as end-products. trans-Xylosidase activity was indicated by the formation of xylobiose and xylotetraose from xylotriose and of xylopentaose and hexaose in addition to biose and triose from xylotetraose. Quantification of hydrolysis products indicated that the enzymes greatest affinity was for the 2nd to 6th β-xylosidic linkages from the terminal of larchwood xylan.
- 社団法人 日本農芸化学会の論文
著者
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SHINMYO Atsuhiko
Department of Biosciences, Nara Institute of Science and Technology
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Okada Hirosuke
Deparment Of Fermentation Technology Osaka University
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Panbangred Watanalai
Department Of Biotechnology Faculty Of Science Mahidol University
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Kinoshita Shinichi
Department Of Chemical Process Engineering Faculty Of Engineering Hokkaido University
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PANBANGRED Watanalai
Department of Fermentation Technology, Faculty of Engineering, Osaka University
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