Degradation of Synthetic Water-Soluble Polymers by Hydroquinone Peroxidase

概要

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The hydroquinone peroxidase of the lignin decolorizing bacterium, Azotobacter beijerinckii HM121 degraded 1 g/l of polyacrylamide (M_r 2,000,000) to small molecules (M_r less than one thousand) in 30 min in the presence of 5 mM hydrogen peroxide and 5μM tetramethylhydroquinone. The two reaction products were purified, and identified as 1-dodecene-2,4,6,8,10-pentacarboxyamide and 1-hexadecene-2,4,6,8,10,12,14-heptacarboxyamide comprising 5 and 7 acrylamide units, respectively. The enzyme also degraded 1 g/l of polyacrylic acid (M_r 450,000) in 1 h, 1 g/l of polyethylene glycol (M_r 4,000,000) in 1 h, and 1 g/l of polyvinyl alcohol (M_r 88,000) for 20 h. The degradation rate decreased with a decrease in the degree of polymerization of the polyethylene glycol from M_r 20,000 to 400. In addition 1 g/l of polyethylene glycol (M_r 4,000,000) was degraded in the absence of tetramethylhydroquinone, although it took 20 h.
社団法人日本生物工学会の論文
1997-09-25