2,3-Butanediol Production by Immobilized Enterobacter aerogenes IAM1133 with _K-Carrageenan

概要

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Enterobacter aerogenes IAM1133 was selected for 2,3-butanediol fermentation among 13 strains known to be 2,3-butanediol producers. This strain produced 11 mg/ml of 2,3-butanediol on 24-hr fermentation with 5% glucose. The cells were entrapped with _K-carrageenan, which was activated in the medium to permit the growth in the matrix. It took one day to obtain the maximum activity of production and this activity was kept stable. The incubation conditions, such as pH, temperature, liquid volume (dissolved oxygen), and glucose concentration, for immobilized cells were not as sensitive as those for native cells. The production of 2,3-butanediol by the immobilized cells was carried out at pH 7 and at 30℃ with 5% glucose and 40 ml of medium in a 100 ml-flask. The long term production by immobilized cells was carried out by sequential replacement culture and continuous culture and they were stable without any loss of activity for at least 10 days. The production of acetoin was decreased by immobilization and the production of 2,3-butanediol by the cells released from the gel was negligible.
社団法人日本生物工学会の論文
1980-04-25