Effects of Pore and Film Diffusion Resistances and Deactivation of Enzyme on the Overall Reaction Rate of Immobilized Enzyme

概要

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A method was developed for investigation of the influence of pore and film diffusional resistances on the overall rate of glucose isomerization reaction subject to enzyme deactivation in the immobilized cells reactor. Two kinds of effectiveness factor, η_D and η_<Df>, were used to illustrate the mass transfer aspects of the system. The actual reaction rate can be expressed in terms of a product of the ideal reaction rate by a multiplier, η_D or η_<Df>. η_D is the multiplier for the ideal rate based on the surface concentration and η_<Df> is based on the bulk concentration. The constant of enzyme deactivation was estimated by numerical analysis of the change in η_D with time obtained experimentally in continuous stirred tank reactor. The surface concentration was estimated by iterative calculation method. A calculation procedure of η_<Df> from kinetic data and physical constants determined by experiments was developed. The satisfactory agreement between the calculated values and the experimental results supports the validity of this theoretical analysis.
社団法人日本生物工学会の論文
1980-10-25