Kinetic Study of Poly-D(-)-3-Hydroxybutyric Acid (PHB) Production and Its Molecular Weight Distribution Control in a Fed-Batch Culture of Alcaligenes eutrophus
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概要
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The kinetics of poly-d-(-)-3-hydroxybutyric acid (PHB) production was studied in Alcaligenes eutrophus H16(ATCC 17699) fed-batch culture using butyric acid as a sole carbon source. In order to control the butyric acid concentration at a steady value, a constant butyric acid feeding policy was developed. At a butyric acid concentration of 3 g/l, pH 8.0,the maximum values of specific PHB production rate, production yield, and PHB content in the cell were obtained. The effects of the cultivation conditions on the molecular weight distribution of PHB wre also studied. The average molecular weight distribution decreased as the butyric acid concentration increased. When a nitrogen source was added to the fermentation medium after adequate PHB accumulation in the cells, not only PHB production but also degradation occurred simultaneously. The degradation rate of low molecular weight polymer was greater than that of high molecular weight polymer, and as a result the average molecular weight shifted to the higher value.
- 社団法人日本生物工学会の論文
- 1993-12-25
著者
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SHIOYA SUTEAKI
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Shimizu Hiroshi
Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osak
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TAMURA Shinji
Department of Internal Medicine, Minoh City Hospital
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Suzuki Shingo
Department Of Bioinformatic Engineering Graduate School Of Information Science And Technology Osaka
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Tamura Shinji
Department Of Biotechnology Faculty Of Engineering Osaka University
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SUGA KEN-ICHI
Department of Biotechnology, Faculty of Engineering, Osaka University
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Suzuki Shingo
Technology Osaka University
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Suga K
Osaka Univ. Osaka Jpn
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Suga K
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Suga K
Osaka Univ. Osaka
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Suga Ken-ichi
Department Of Biological Science And Technology Science University Of Tokyo
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Tamura Shinji
Department Of Applied Chemistry Faculty Of Engineering And Handai Frontier Research Center Osaka Uni
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Shimizu Hiroshi
Department Of Bioinformatic Engineering Graduate School Of Information Science And Technology Osaka
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Shioya S
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Shioya Suteaki
Department Of Biotechnology Graduate School Of Engineering Osaka University:(present Office)departme
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Shioya Suteaki
Department Of Biotechnology Faculty Of Engineering Osaka University
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Shioya Suteaki
Department Of Fermentation Technology Osaka University
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Shimizu H
Osaka Univ. Osaka Jpn
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Shimizu Hiroshi
Department Of Animal Science Faculty Of Agriculture Hokkaido University
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SHIMIZU HIROSHI
Department of Anatomy, School of Medicine, Tokushima University
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