New Strategy for Enhancement of Microbial Viability in Simulated Gastric Conditions Based on Display of Starch-Binding Domain on Cell Surface(BIOCHEMICAL ENGINEERING)
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概要
- 論文の詳細を見る
The C-terminal region of the peptidoglycan hydrolase(CPH) of Lactococcus lactis IL1403 fused to the linker region and the starch-binding domain(SBD) of the α-amylase of Streptococcus bovis 148 was produced intracellularly in Escherichia coll. The fusion protein(CPH-SBD) was able to bind to the cell surface of Lactobacillus easel NRRL B-441 and to corn starch. Therefore, adhesion of cells to corn starch was mediated by the fusion protein. At a cell density of 10^9 cfu/ml and a starch concentration of 5 mg/ml, CPH-SBD-displaying L. casei cells aggregated with corn starch, whereas the free cells of L. casei did not form any aggregates with corn starch. After incubation in simulated gastric juice(pH 3.0, 1 h), the survival percentages of free cells, amylose-coated free cells, and free cells mixed with corn starch were 0.074%, 7.2%, and 3.1% respectively. When CPH-SBD-displaying bacteria aggregated with corn starch, their survival percentage was 8% higher than that of free cells mixed with corn starch. The survival of the amylose-coated CPH-SBD-displaying L. casei cells was comparable to that of amylose-coated free cells, whereas the survival percentage of amylose-coated aggregates of CPH-SBD-displaying bacteria with corn starch was 28% higher than that of amylose-coated mixture of free cells with corn starch. These results demonstrate the potential usefulness of the cell-surface display technique for enhancement of the delivery of viable microorganisms to the intestinal tract.
- 社団法人日本生物工学会の論文
- 2008-05-25
著者
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KATAKURA YOSHIO
Department of Biotechnology, Graduate School of Engineering, Osaka University
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SHIOYA SUTEAKI
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Yasuhara Kazuo
Divisions Of Toxicology National Institute Of Hygienic Sciences
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安原 加壽雄
San-ei Gen F.f.i. Inc.
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Katakura Yoshio
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Katakura Yoshio
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Tarahomjoo Shirin
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Suzuki Shingo
Technology Osaka University
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Kamimura Yasuhiro
Safety Evaluation Lab. Yoshitomi Pharm.
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Tarahomjoo Shirin
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Yoshikawa Katsunori
Department Of Bioinformatic Engineering Graduate School Of Information Science And Technology Osaka
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Kurokawa Y
Division Of Cellular & Molecular Toxicology National Institute Of Health Sciences
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Kodama Y
Biological Safety Research Center National Institute Of Health Science
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Shioya S
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Shioya Suteaki
Department Of Biotechnology Graduate School Of Engineering Osaka University:(present Office)departme
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Shioya Suteaki
Technology Osaka University
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Shioya Suteaki
Department Of Biotechnology Faculty Of Engineering Osaka University
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Kawasaki Yasushi
National Institute Of Health Sciences
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Kashida Y
Laboratory Of Veterinary Pathology Tokyo University Of Agriculture And Technology
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Kawasaki Yasushi
Div.of Toxicol. National Institute Of Health Sciences
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Katakura Yoshio
Department Of Bioinformatic Engineering Graduate School Of Information Science And Technology Osaka University
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Yasuhara Kazuo
Division Of Toxicology Biological Safety Research Center National Institute Of Hygienic Sciences
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