Physiological Analysis of Yeast Cells by Flow Cytometry during Serial-Repitching of Low-Malt Beer Fermentation(BREWING AND FOOD TECHNOLOGY)
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概要
- 論文の詳細を見る
At the end of beer brewing fermentation, yeast cells are collected and repitched for economical reasons. Although it is generally accepted that the physiological state of inoculated yeast cells affects their subsequent fermentation performance, the effect of serial-repitching on the physiological state of such yeast cells has not been well clarified. In this study, the fermentation performance of yeast cells during serial-repitching was investigated. After multiple repitchings, the specific growth rate and maximum optical density (OD_<660>) decreased, and increases in isoamyl alcohol, which causes an undesirable flavor, and residual free amino acid nitrogen (FAN) concentrations were observed. The physiological state of individual cells before inoculation was characterized by flow cytometry using the fluorescent dyes dehydrorhodamine 123 (DHR) and bis-(1,3-di-butylbarbituric acid) trimethine oxonol (OXN). The fluorescence intensities of DHR, an indicator of reactive oxygen species (ROSs), and OXN, which indicates membrane potential, gradually increased as the number of serial-repitching cycles increased. Fluorescence intensity correlated strongly with cell growth. The subsequent fermentation performance can be predicted from this correlation.
- 社団法人日本生物工学会の論文
- 2007-05-25
著者
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SHIOYA SUTEAKI
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Shimizu Hiroshi
Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osak
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Suzuki Shingo
Department Of Bioinformatic Engineering Graduate School Of Information Science And Technology Osaka
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Suzuki Shingo
Technology Osaka University
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Shimizu Hiroshi
Department Of Bioinformatic Engineering Graduate School Of Information Science And Technology Osaka
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Kobayashi Michiko
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Shioya S
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Shioya Suteaki
Technology Osaka University
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Shioya Suteaki
Department Of Biotechnology Faculty Of Engineering Osaka University
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Shimizu Hiroshi
Department Of Animal Science Faculty Of Agriculture Hokkaido University
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Kobayashi Michiko
Department Of Biological Science Faculty Of Science Yamaguchi University
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Shimizu Hiroshi
Department Of Bioinformatic Engineering Graduate School Of Information Science And Technology Osaka University
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SHIMIZU HIROSHI
Department of Anatomy, School of Medicine, Tokushima University
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