Strategies for Reducing Supplemental Medium Cost in Bioethanol Production from Waste House Wood Hydrolysate by Ethanologenic Escherichia coli : Inoculum Size Increase and Coculture with Saccharomyces cerevisiae(BIOCHEMICAL ENGINEERING)

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In this paper, we report a simultaneous realization of both efficient ethanol production and saving medium nutrient(corn steep liquor [CSL]) during bioethanol fermentation of overliming-treated hydrolysate of waste house wood(WHW) using ethanologenic Escherichia coli KO11. In cultivation using WHW hydrolysate supplemented with 4%(v/v) CSL and 0.2 g-dry cell weight(DCW)/l E. coli KO11 cells, the overall ethanol yield reached 84% of the theoretical value at 61 h. When we conducted the cultivation with 1% CSL to reduce the supplemental medium cost, the overall ethanol yield remained in the range of 66-72% even at 90 h. We proposed two alternative methods for increasing the overall yield even with 1% CSL. The first method involved increasing the inoculum size of E. coli KO11 up to 0.8 g-DCW/l, where 83% of the overall yield was attained at 60 h of cultivation. The second method involved the coculture of 0.2 g-DCW/l E. coli KO11 together with 0.02 g-DCW/l of Saccharomyces cerevisiae TJ1, and the overall yield reached 81% at 47 h of cultivation.
社団法人日本生物工学会の論文
2008-02-25