Simulation Model for Predicting Limit of Detection and Range of Quantitation of Competitive Enzyme-Linked Immunosorbent Assay(BIOCHEMICAL ENGINEERING)
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概要
- 論文の詳細を見る
The limit of detection (LOD) and range of quantitation (ROQ) of competitive enzyme-linked immunosorbent assay (ELISA) were determined from a model describing the calibration curve and precision profile of the assay. The calibration curve is given by solving the differential equations describing the change in the concentrations of an antigen-antibody complex and an enzyme-conjugated antigen-antibody complex by a Runge-Kutta method. The precision profile is described in terms of possible error sources such as the pipetting volumes of the analyte, enzyme-conjugated antigen, antibody and substrate solutions, calibration curve and inherent absorbances between the wells in an ELISA plate. An appropriate concentration of the enzyme-conjugated antigen that balances a low detection limit and sufficient color development was found to be in a narrow range, which is consistent with the empirical rule. The optimum conditions for competitive ELISA using an antibody with a kinetic property can be designed from our model.
- 社団法人日本生物工学会の論文
- 2007-05-25
著者
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KATAKURA YOSHIO
Department of Biotechnology, Graduate School of Engineering, Osaka University
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SHIOYA SUTEAKI
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Yasuhara Kazuo
Divisions Of Toxicology National Institute Of Hygienic Sciences
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安原 加壽雄
San-ei Gen F.f.i. Inc.
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CHOI Dong
Department of Ophthalmology, Hallym University
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Suzuki Shingo
Department Of Bioinformatic Engineering Graduate School Of Information Science And Technology Osaka
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Katakura Yoshio
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Katakura Yoshio
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Ninomiya Kazuaki
Department Of Biotechnology Graduate School Of Engineering Osaka University:(present Office)institut
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MATSUDA Rieko
National Institute of Health Sciences
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Hayashi Yuzuru
National Institute of Health Sciences
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Suzuki Shingo
Technology Osaka University
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Kamimura Yasuhiro
Safety Evaluation Lab. Yoshitomi Pharm.
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Yoshikawa Katsunori
Department Of Bioinformatic Engineering Graduate School Of Information Science And Technology Osaka
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Kurokawa Y
Division Of Cellular & Molecular Toxicology National Institute Of Health Sciences
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Kodama Y
Biological Safety Research Center National Institute Of Health Science
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Choi Dong
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Shioya S
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Shioya Suteaki
Department Of Biotechnology Graduate School Of Engineering Osaka University:(present Office)departme
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Shioya Suteaki
Technology Osaka University
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Shioya Suteaki
Department Of Biotechnology Faculty Of Engineering Osaka University
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Kawasaki Yasushi
National Institute Of Health Sciences
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Kashida Y
Laboratory Of Veterinary Pathology Tokyo University Of Agriculture And Technology
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Kawasaki Yasushi
Div.of Toxicol. National Institute Of Health Sciences
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Choi Dong
Department Of Biological Education Chonnam National University
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Matsuda Rieko
Div. Of Foods National Inst. Of Health Sciences
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Katakura Yoshio
Department Of Bioinformatic Engineering Graduate School Of Information Science And Technology Osaka University
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Yasuhara Kazuo
Division Of Toxicology Biological Safety Research Center National Institute Of Hygienic Sciences
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