Molecular Pathological Analysis of Apoptosis Induced in Testicular Germ Cells after a Single Administration of Mono-(2-ethylhexyl) phthalate to F344 Rats
スポンサーリンク
概要
- 論文の詳細を見る
Mono-(2-ethylhexyl) phthalate (MEHP) is known to cause germ cell apoptosis in the testis of rats as a consequence of Sertoli cell damage. In the present study, a single dose of 2,000 mg/kg body weight MEHP was orally administered to Fischer 344 rats and the molecular apoptosis pathway was surveyed. Histopathological examination, TUNEL assay and immunohistochemical analysis of cleaved caspase-3 were performed to assess the reproducibility of apoptosis. In addition, immunohistochemical analysis and RT-PCR to determine the parameters of the apoptosis pathway were performed. Histopathological examination showed that from 6 to 12 hours after MEHP treatment there was detachment of germ cells that had eosinophilic cytoplasm and pyknotic nuclei. A TUNEL assay detected a significant increase in TUNEL-positive cells at 12 hours after treatment. Immunohistochemistry showed some of the cytoplasm of germ cells was positive for cleaved caspase-3 at 12 hours after treatment. In immunohistochemistry of the Fas/Fas ligand (FasL) apoptosis pathway, the cytoplasm of germ cells was found to be occasionally positive for Fas at 12 hours after treatment, and the expression of the Fas gene in RT-PCR had significantly increased at 12 hours after treatment. RT-PCR of the mitochondrial apoptosis pathway revealed a significant increase in the expression of Bax and Bcl-2 genes in the treated group at 12 hours after treatment. These results strongly suggest that both the Fas/FasL and mitochondrial pathways are involved in germ cell apoptosis induced by MEHP.
- 日本毒性病理学会の論文
- 2006-12-25
著者
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MITSUMORI Kunitoshi
Laboratory of Veterinary Pathology, Tokyo University of Agriculture and Technology
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Mitsumori Kunitoshi
Laboratory Of Veterinary Pathology Tokyo University Of Agriculture And Technology
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Mitsumori Kunitoshi
東京大学医科学研究所 癌細胞シグナル分野
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Mitsumori Kunitoshi
Toxicology Division Institute Of Environmental Toxicology
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Mitsumori Kunitoshi
国立医薬品食品衛生研究所 病理部
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YAMAZAKI Masaki
Laboratory of Veterinary of Pathology, Tokyo University of Agriculture and Technology
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MOTO Mitsuyoshi
Laboratory of Veterinary of Pathology, Tokyo University of Agriculture and Technology
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Mitsumori K
Laboratory Of Veterinary Pathology Tokyo University Of Agriculture And Technology
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Yasuhara Kazuo
Divisions Of Toxicology National Institute Of Hygienic Sciences
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Yasuhara Kazuo
Division Of Pathology National Institute Of Health Sciences
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Muguruma Masako
Laboratory of Veterinary Pathology, Tokyo University of Agriculture and Technology
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TAKAHASHI Miwa
Laboratory of Veterinary Pathology, Tokyo University of Agriculture and Technology
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KASHIDA Yoko
Division of Medicinal Chemistry and Pharmacognosy, Ohio State University
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Moto Mitsuyoshi
東京農工大学 獣医病理学
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Moto Mitsuyoshi
Laboratory Of Veterinary Pathology Tokyo University Of Agriculture And Technology
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Muguruma Masako
東京農工大学 獣医病理学
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Muguruma Masako
Laboratory Of Veterinary Pathology Tokyo University Of Agriculture And Technology
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Kodama Yukio
Division Of Pathology National Institute Of Health Sciences
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Kitamura Y
Division Of Pathology National Institute Of Health Sciences
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安原 加壽雄
San-ei Gen F.f.i. Inc.
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Mitsumori Kunitoshi
Department Of Pathology National Institute Of Health Sciences
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Mitsumori Kunitoshi
Laboratory Of Veterinary Pathology Division Of Animal Science Institute Of Symbiotic Science And Tec
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Takahashi Miwa
Division Of Pathology National Institute Of Health Sciences
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Yamazaki Masaki
Laboratory Of Veterinary Pathology Faculty Of Agriculture Tokyo University Of Agriculture And Techno
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Kamimura Yasuhiro
Safety Evaluation Lab. Yoshitomi Pharm.
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Yoshikawa Katsunori
Department Of Bioinformatic Engineering Graduate School Of Information Science And Technology Osaka
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Matsumoto K
Kyushu Univ. Fukuoka Jpn
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Matsumoto K
Drug Safety Research Laboratories Pharmaceutical Research Division Takeda Chemical Industries Ltd.:l
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Kurokawa Y
Division Of Cellular & Molecular Toxicology National Institute Of Health Sciences
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Kodama Y
Biological Safety Research Center National Institute Of Health Science
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三森 国敏
Division Of Pathology National Institute Of Health Sciences
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Kawasaki Yasushi
National Institute Of Health Sciences
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Kashida Y
Laboratory Of Veterinary Pathology Tokyo University Of Agriculture And Technology
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Kodama Yukio
Cel. Mol. Tox. Div. Nihs
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Matsumoto K
Laboratory Of Veterinary Pathology Tokyo University Of Agriculture And Technology
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Takahashi Miwa
Laboratory Of Veterinary Pathology Faculty Of Agriculture Tokyo University Of Agriculture And Techno
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Katakura Yoshio
Department Of Life Science And Bioengineering Faculty Of Chemistry Materials And Bioengineering Kans
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Kashida Yoko
Division Of Medicinal Chemistry And Pharmacognosy Ohio State University
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Yasuhara Kazuo
Division Of Toxicology Biological Safety Research Center National Institute Of Hygienic Sciences
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Katakura Yoshio
Department Of Biotechnology Faculty Of Engineering Osaka University
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