Preparation and Characterization of Liposomal Microencapsulated Poly-γ-glutamic Acid for Prevention of Ca-Phosphate Precipitation under Intestinal Environment
スポンサーリンク
概要
- 論文の詳細を見る
Poly-γ-glutamic acid (γ-PGA) from Bacillus subtilis (natto) with a high molecular weight (Av. M.W.=350,000) effectively inhibited the formation of insoluble calcium phosphate at Ca/P=0.5 (12.5 mM/25 mM) under the condition of 37°C and pH 7.4. Since decrease in inhibitory activity in γ-PGA was observed after the treatment by simulated gastric fluid (pH 1.2, 0.5% pepsin), γ-PGA was encapsulated by egg lecithin liposome to keep the inhibitory activity under gastric conditions. Encapsulation was done by dehydration-rehydration (DR) and reverse-phase-vesicle (REV) methods. Higher encapsulation efficiency (57.0%) was achieved by DR method. Turbidity measurement at 450 nm indicated the high stability of DR microcapsule, prepared with trehalose, against gastric juice. Encapsulated γ-PGA by DR method showed 93.3% residual inhibitory activity after the gastric juice treatment.
- 社団法人 日本食品科学工学会の論文
- 2004-05-01
著者
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Mitsumori Kunitoshi
東京大学医科学研究所 癌細胞シグナル分野
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Mitsumori Kunitoshi
Toxicology Division Institute Of Environmental Toxicology
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Mitsumori Kunitoshi
Department Of Pathology National Institute Of Health Sciences
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ISHIKAWA Hiroya
Laboratory of Food Biotechnology, Department of Bioscience and Biotechnology, Division of Bioresourc
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MATSUMOTO Kiyoshi
Laboratory of Food Biotechnology, Department of Bioscience and Biotechnology, Division of Bioresourc
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Matsumoto K
Drug Safety Research Laboratories Pharmaceutical Research Division Takeda Chemical Industries Ltd.:l
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三森 国敏
Division Of Pathology National Institute Of Health Sciences
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Matsumoto K
Laboratory Of Veterinary Pathology Tokyo University Of Agriculture And Technology
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Hayakawa I
Laboratory Of Food Process Engineering Division Of Food Biotechnology Department Of Bioscience And B
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SHIMODA Yoshihiro
Laboratory of Food Analysis, Division of Food Biotechnology, Department of Bioscience and Biotechnol
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Shimoda Yoshihiro
Laboratory Of Food Analysis Division Of Food Biotechnology Department Of Bioscience And Biotechnolog
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Ishikawa Hiroya
Laboratory Of Food Analysis Division Of Food Biotechnology Department Of Bioscience And Biotechnolog
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Ishikawa Hiroya
Department Of Bioscience And Biotechnology Faculty Of Agriculture Graduate School Of Kyushu Universi
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Matsumoto Kiyoshi
Laboratory Of Food Analysis Division Of Food Biotechnology Department Of Bioscience And Biotechnolog
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