Cytokeratin 8/18 Is a Useful Immunohistochemical Marker for Hepatocellular Proliferative Lesions in Mice
スポンサーリンク
概要
- 論文の詳細を見る
In order to clarify whether cytokeratin (CK) 8/18 is a useful immunohistochemical marker for hepatocellular proliferative lesions in mice, partially hepatectomized male ICR mice were given 0.6% piperonyl butoxide (PBO) for 8 (Experiment I) or 25 weeks (Experiment II) after N-diethylnitrosamine (DEN) initiation treatment, and the livers were subjected to histological examinations on hematoxylin and eosin (HE) stained sections, CK8/18 immunohistochemistry and gamma-glutamyl transpeptidase (GGT) histochemistry. In Experiment I, the multiplicity of hepatocellular foci in paraffin-embedded sections which were observed in HE-stained sections and positive for CK8/18 was 10.17 and 18.50, respectively, while that of hepatocellular foci in frozen sections which were observed in HE-stained sections and positive/negative for GGT was 6.17 and 8.17, respectively. In Experiment II, the total multiplicity of hepatocellular foci in paraffin-embedded sections which were observed in HE-stained sections and positive/negative for CK8/18 was 4.47 and 23.17, respectively, while that of hepatocellular foci in frozen sections which were observed in HE-stained sections and positive/negative for GGT was 2.50 and 3.50, respectively. Most of the hepatocellular adenomas and carcinomas observed in HE-stained sections were positive for CK8/18, but some of the adenomas were negative for CK8/18. These findings indicate that more hepatocellular proliferative lesions can be detected in CK8/18 immunohistochemistry in addition to those observed in HE-stained sections, and suggest that CK8/18 may become a useful immunohistochemical marker for detecting hepatocellular proliferative lesions in mice.
著者
-
KAWAI Masaomi
Laboratory of Veterinary Pathology, Tokyo University of Agriculture and Technology
-
SAEGUSA Yukie
Laboratory of Veterinary Pathology, Tokyo University of Agriculture and Technology
-
KEMMOCHI Sayaka
Laboratory of Veterinary Pathology, Tokyo University of Agriculture and Technology
-
HARADA Tomoaki
Laboratory of Veterinary Pathology, Tokyo University of Agriculture and Technology
-
SHIBUTANI Makoto
Laboratory of Veterinary Pathology, Tokyo University of Agriculture and Technology
-
MITSUMORI Kunitoshi
Laboratory of Veterinary Pathology, Tokyo University of Agriculture and Technology
-
Mitsumori Kunitoshi
Laboratory Of Veterinary Pathology Division Of Animal Science Institute Of Symbiotic Science And Tec
-
Saegusa Yukie
Laboratory Of Veterinary Pathology Tokyo University Of Agriculture And Technology
-
Kawai Masaomi
Laboratory Of Veterinary Pathology Tokyo University Of Agriculture And Technology
-
Harada Tomoaki
Laboratory Of Veterinary Pathology Tokyo University Of Agriculture And Technology
-
Shibutani Makoto
Laboratory Of Veterinary Pathology Tokyo University Of Agriculture And Technology
-
Shimamoto Keisuke
Laboratory of Veterinary Pathology, Tokyo University of Agriculture and Technology
-
KAWAWI Masaomi
Laboratory of Veterinary Pathology, Tokyo University of Agriculture and Technology
-
Kemmochi Sayaka
Laboratory Of Veterinary Pathology Tokyo University Of Agriculture And Technology
-
Shimamoto Keisuke
Laboratory Of Veterinary Pathology Tokyo University Of Agriculture And Technology
関連論文
- The threshold dose for liver tumor promoting effects of dicyclanil in ICR mice
- A Mixed Apocrine Gland Tumor with Metastases to the Bone and Bone Marrow in a Miniature Poodle
- Enhancement of esophageal carcinogenesis in acid reflux model rats treated with ascorbic acid and sodium nitrite in combination with or without initiation
- Lack of Modifying Effects of Combined Treatment of t-butylhydroquinone and Sodium Nitrite on Forestomach Carcinogenesis in rasH2 Mice Initiated with N-ethyl-N-nitrosourea
- Lack of Initiating Activity of Kojic Acid on Hepatocarcinogenesis in F344 Rats
- Tumorigenic Susceptibility of Catechol on the Gastric Mucosa in rasH2 Mice
- Lack of Modifying Effect of Glycerol in Pulmonary Carcinogenesis in rasH2 Mice Induced by Urethane or 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK)
- Inhibiting Effects of Ethinylestradiol on N-ethyl-M-nitrosourea-Initiated Uterine Carcinogenesis in Transgenic Mice Carrying a Human Prototype C-Ha-ras Gene (rasH2 mice) (CARCINOGENICITY) (GENERAL SESSION BY POSTER PRESENTATION) (Proceedings of the 30th A
- Promoting Effects of Ethinylestradiol but not Atrazine on N-ethyl-N-nitrosourea-induced Uterine Carcinogenesis in ICR Mice
- Modifying Effects of 2,6-Dimethylaniline on Nasal Carcinogenesis in RasH2 Mice Initiated with 4-Methylnitrosamino)-1-(3-pyridyl)-1-butanone
- Lack of Modifying Effects of 2,6-Dimethylaniline on Lung Carcinogenesis in Transgenic Mice Carrying Human Prototype c-Ha-ras Gene Initiated with 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone
- Inhibiting Effects of Ethinylestradiol on N-ethyl-M-nitrosourea-Initiated Uterine Carcinogenesis in Transgenic Mice Carrying a Human Prototype C-Ha-ras Gene (rasH2 mice) (CARCINOGENICITY) (GENERAL SESSION BY POSTER PRESENTATION) (Proceedings of the 30th A
- Crosstalk between PTEN/Akt2 and TGFβ signaling involving EGF receptor down-regulation during the tumor promotion process from the early stage in a rat two-stage hepatocarcinogenesis model
- A Case Report of a Cerebellar Neuroblastoma in a p53 Null Mutation Mouse(Pathology)
- Suppressive effect of Siraitia grosvenorii extract on dicyclanil-promoted hepatocellular proliferative lesions in male mice
- Modifying effect of Siraitia grosvenori extract on piperonyl butoxide-promoted hepatocarcinogenesis in rats
- Concurrent administration of ascorbic acid enhances liver tumor-promoting activity of kojic acid in rats
- Reactive Oxygen Species Are Possibly Involved in the Mechanism of Flumequine-Induced Hepatocarcinogenesis in Mice
- Molecular Analysis on the Possible Mechanism of β-Naphthoflavone-Induced Hepatocarcinogenesis in Rats
- Molecular Pathological Analysis of Apoptosis Induced in Testicular Germ Cells after a Single Administration of Mono-(2-ethylhexyl) phthalate to F344 Rats
- Mechanistic study on hepatocarcinogenesis induced by dicyclanil in mice : possible involvement of oxidative stress(Proceedings of the 32nd Annual Meeting)
- Induction of Hepatocellular Proliferative Lesions in CBA Mice by a 26-week Dietary Administration of Kojic Acid
- Absence of DNA Damage in Comet Assay and Liver Initiation Activity of Cinnamaldehyde
- Modifying Effects of Genistein, 4-Nonylphenol and Methoxychlor on N-ethyl-N-nitrosourea-induced Uterine Carcinogenesis in RasH2 Transgenic Mice Harboring a Human Prototype C-Ha-ras Gene
- Inhibiting Effects of Ethinylestradiol on N-ethyl-M-nitrosourea-Initiated Uterine Carcinogenesis in Transgenic Mice Carrying a Human Prototype C-Ha-ras Gene (rasH2 mice) (CARCINOGENICITY) (GENERAL SESSION BY POSTER PRESENTATION) (Proceedings of the 30th A
- Inhibiting Effects of Ethinylestradiol on N-ethyl-M-nitrosourea-Initiated Uterine Carcinogenesis in Transgenic Mice Carrying a Human Prototype C-Ha-ras Gene (rasH2 mice) (CARCINOGENICITY) (GENERAL SESSION BY POSTER PRESENTATION) (Proceedings of the 30th A
- Analysis of Gene Expression Profile on Uterine Tumorigenesis Initiated with N-ethyl-N-nitrosourea and Inhibited by Ethinylestradiol in rasH2 Mice
- THIRTEEN-WEEK REPEATED DOSE TOXICITY OF RICE BRAN GLYCOSPHINGOLIPID IN WISTAR HANNOVER (GALAS) RATS
- THIRTEEN-WEEK REPEATED DOSE TOXICITY STUDY OF WORMWOOD (ARTEMISIA ABSINTHIUM) EXTRACT IN RATS
- Inhibiting Effects of Ethinylestradiol on N-ethyl-M-nitrosourea-Initiated Uterine Carcinogenesis in Transgenic Mice Carrying a Human Prototype C-Ha-ras Gene (rasH2 mice) (CARCINOGENICITY) (GENERAL SESSION BY POSTER PRESENTATION) (Proceedings of the 30th A
- Investigation on liver initiation activity and DNA damages of flumequine in mice.(GENERAL SESSION BY ORAL PRESENTATION)(CARCINOGENICITY)
- Enhancing effects of carbon tetrachloride on in vivo mutagenicity in the liver of mice fed 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx)
- Effects of co-treatment of dextran sulfate sodium and MeIQx on genotoxicity and possible carcinogenicity in the colon of p53-deficient mice
- A Case Report of an Uncommon Sex-Cord Stromal Tumor consisted of Luteal and Sertoli Cells in a Spayed Bitch
- O-13 Genotoxic potential in vitro and liver initiation activity of norfloxacin(CARCINOGENICITY)(GENERAL SESSION BY ORAL PRESENTATION)(Proceedings of the 31st Annual Meeting)
- O-12 Molecular pathological analyses on hepatocarcinogenesis induced by dicyclanil in mouse two-stage liver carcinogenesis model(CARCINOGENICITY)(GENERAL SESSION BY ORAL PRESENTATION)(Proceedings of the 31st Annual Meeting)
- Time Course of Expression of 7,12-Dimethylbenz[a]anthracene-induced CYP1A1 and CYP1B1 mRNA and Protein in Rat Liver
- ABSENCE OF IN VIVO GENOTOXICITY AND LIVER INITIATION ACTIVITY OF DICYCLANIL
- Cellular distributions of molecules with altered expression specific to thyroid proliferative lesions developing in a rat thyroid carcinogenesis model
- Pathological assessment of the nervous and male reproductive systems of rat offspring exposed maternally to acrylamide during the gestation and lactation periods-a preliminary study
- Ultrastructural Morphology of Motor Endplate Neurotoxicities in Rats
- Evaluation of carcinogenicity on chemicals using transgenic mice
- P11-10 Kidney damages induced by tetrabromobisphenol A in newborn rats
- Deficiency of antiproliferative family protein Ana correlates with development of lung adenocarcinoma
- RELATIONSHIP BETWEEN TOXICITY AND CADMIUM ACCUMULATION IN RATS GIVEN LOW AMOUNTS OF CADMIUM CHLORIDE OR CADMIUM-POLLUTED RICE FOR 22 MONTHS
- Lack of Modifying Effect of Arctiin on ENU-Induced Uterine Carcinogenesis in ICR Mice
- Cytokeratin 8/18 Is a Useful Immunohistochemical Marker for Hepatocellular Proliferative Lesions in Mice
- Classification of Renal Proliferative Lesions in Rats and/or Mice and Their Diagnostic Problems : Report from the Working Group of the Japanese Society of Toxicologic Pathology
- POSSIBLE MECHANISM ON ENHANCED CARCINOGENESIS OF GENOTOXIC CARCINOGENS AND UNSOLVED MECHANISMS ON LESSER CARCINOGENIC SUSCEPTIBILITY TO SOME CARCINOGENS IN RASH2 MICE
- Dual antitumor mechanisms of Notch signaling inhibitor in a T-cell acute lymphoblastic leukemia xenograft model
- A Neuroendocrine Carcinoma of Undetermined Origin in a Dog
- No Modifying Effect of Antioxidant N-Acetyl-L-Cysteine on Fenofibrate-induced Hepatocarcinogenesis in Rats
- Carcinogenic potential of alizarin and rubiadin, components of madder color, in a rat medium-term multi-organ bioassay
- Gene Expression Profiling and Cellular Distribution of Molecules with Altered Expression in the Hippocampal CA1 Region after Developmental Exposure to Anti-Thyroid Agents in Rats
- Preventive Effects of Calcitriol on the Development of Capsular Invasive Carcinomas in a Rat Two-Stage Thyroid Carcinogenesis Model
- Spontaneous Renal Mixed Epithelial and Stromal Tumor (MEST) in a Beagle
- Liver tumor promoting effect of etofenprox in rats and its possible mechanism of action
- Antioxidant N-acetyl-L-cysteine (NAC) supplementation reduces reactive oxygen species (ROS)-mediated hepatocellular tumor promotion of indole-3-carbinol (I3C) in rats
- Threshold dose of liver tumor promoting effect of β-naphthoflavone in rats
- Liver tumor promoting effect of omeprazole in rats and its possible mechanism of action
- Twenty-six-week oral toxicity of diheptyl phthalate with special emphasis on its induction of liver proliferative lesions in male F344 rats
- Promoting effects of carminic acid-enriched cochineal extracts on capsular invasive thyroid carcinomas through targeting activation of angiogenesis in rats
- Adolescent hyperactivity of offspring after maternal protein restriction during the second half of gestation and lactation periods in rats
- Complex Apocrine Carcinoma with Dominant Myoepithelial Proliferation in a Dog
- Protective Effect of Stachybotrys microspora Triprenyl Phenol-7on the Deposition of IgA to the Glomerular Mesangium in Nivalenol-induced IgA Nephropathy Using BALB/c Mice
- Enhanced liver tumor promotion but not liver initiation activity in rats subjected to combined administration of omeprazole and β-naphthoflavone
- A Neuroendocrine Carcinoma of Undetermined Origin in a Dog
- A Mixed Apocrine Gland Tumor with Metastases to the Bone and Bone Marrow in a Miniature Poodle
- No Modifying Effect of Antioxidant N-Acetyl-L-Cysteine on Fenofibrate-induced Hepatocarcinogenesis in Rats
- A Case Report of a Renal Mixed Epithelial and Stromal Tumor in a Heterozygous S1P2 Receptor Deficient Mouse(Pathology)
- Neuropathological Evaluation of Acrylamide- and 3,3'-Iminodipropionitrile-Induced Neurotoxicity in a Rat 28-Day Oral Toxicity Study-Collaborative Project for Standardization of Test Procedures and Evaluation of Neurotoxicity
- Aberrant activation of ubiquitin D at G_2 phase and apoptosis by carcinogens that evoke cell proliferation after 28-day administration in rats
- Induction of ovarian toxicity in a subchronic oral toxicity study of citrinin in female BALB/c mice
- Fluctuations in cell proliferation, apoptosis, and cell cycle regulation at the early stage of tumor promotion in rat two-stage carcinogenesis models
- Environmental Agents, Endocrine Disrupting Chemicals and Rat Thyroid Carcinogenesis
- Enhanced liver tumor promotion activity in rats subjected to combined administration of phenobarbital and orphenadrine
- Liver tumor promoting effect of orphenadrine in rats and its possible mechanism of action including CAR activation and oxidative stress
- Detection of initiating potential of non-genotoxic carcinogens in a two-stage hepatocarcinogenesis study in rats
- Suppressive effect of liver tumor-promoting activities in rats subjected to combined administration of phenobarbital and piperonyl butoxide