Microaeration Enhances Productivity of Bioethanol from Hydrolysate of Waste House Wood Using Ethanologenic Escherichia coli KO11(ENVIRONMENTAL BIOTECHNOLOGY)

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This is the first study showing the successful application of waste house wood(WHW) to the pilot-scale production of bioethanol by hydrolysis using diluted acid and fermentation using the ethanologenic recombinant Escherichia coli KO11. The major sugars in the WHW hydrolysate were glucose, mannose and xylose; the percentages were approximately 35%, 35% and 20%(w/w), respectively. In anaerobic fermentation using a 5-7 reactor in which the oxygen transfer rate(OTR) was Ommol/(l・h), KO11 consumed only 25% of the xylose in the WHW hydrolysate over the examined fermentation time of 100 h; however, hexoses such as glucose and mannose were consumed completely. Microaeration at an OTR of 4mmol/(l・h) enhanced the xylose utilization ratio of KO11 to 100%, at which the ethanol concentration was 35.4 g/l and the ethanol yield was 0.42, although the maximum ethanol concentrations were 28.8 and 26.6 g/l at OTRs of Ommol/(l・h) and 15mmol/(/-h), respectively. Moreover, this microaerobic fermentation at OTR of 4mmol/(l・h) was applied to 1000-l scale bioethanol production using the WHW hydrolysate. The xylose utilization ratio reached 100% and the ethanol yield was determined to be 0.45 for a 63-h fermentation, which were comparable to those obtained from the laboratory-scale fermentation.
社団法人日本生物工学会の論文
2007-04-25