Mole Fraction Control of Poly(3-Hydroxybutyric-Co-3-Hydroxyvaleric Acid in Fed-Batch Culture of Alcaligenes eutrophus
スポンサーリンク
概要
- 論文の詳細を見る
The aims of this study were to investigate the reaction kinetics of production of poly(3-hydroxybutyric-co-3-hydroxyvaleric) acid, P(HB-co-HV), to control its mole fraction in a fermnetor, and to develop an optimal copolymer production system using fed-batch cultures of Alcaligenes eutrophus ATCC 17699. In this study, butyric and valeric acids were utilized as carbon sources. In order to keep the concentrations of these fatty acids constant, the well-organized fed-batch experiments were performed using a 5 l jar fermentor equipped with a temperature and pH control system. Mole fraction and sequence distribution of monomeric units in a copolymer, 3-hydroxybutyric acid (HB) and 3-hydroxyvaleric acid (HV), were determined by ^1H NMR and ^<13>C NMR spectroscopy, respectively. The specific production rate of P(HB-co-HV) was lower than that of PHB when the total fatty acid concentration in the medium was 3.0 g/l, and it was constant and independent of the mole fraction of valeric acid at total fatty acid concentrations less than 0.3 g/l. The mole fraction of HV units in copolymers (0-40%) was linearly related to the mole fraction of valeric acid in the medium. Thus, the mole fractin of HV units in copolymer can be controlled within the range of 0-40 mol% by adjusting the mole fraction of butyric acid in the feeding medium. Moreover, for P(HB-co-HV) with a given mole fraction of HB and HV units, the most economical feeding strategy in terms of achieving the maximum reaction rate involved adjusting the total fatty acid concentration. From the sequence distribution of diads and triads of HB and HV units, P(HB-co-HV) with a 0-40% mole fraction of HV was concluded to be a random copolymer. The weight average molecular weight of P(HB-co-HV) measured by the GPC method changed from 0.77×10^6 to 3.4×10^6. This range is of the same order of magnitude as the homopolymer, PHB.
- 社団法人日本生物工学会の論文
- 1996-05-25
著者
-
SHIOYA SUTEAKI
Department of Biotechnology, Graduate School of Engineering, Osaka University
-
Ishihara Yoshiyuki
Department of Cardiology, Gifu University School of Medicine
-
Shimizu Hiroshi
Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osak
-
Suzuki Shingo
Department Of Bioinformatic Engineering Graduate School Of Information Science And Technology Osaka
-
Suzuki Shingo
Technology Osaka University
-
Ishihara Yoshiyuki
Department Of Biotechnology Faculty Of Engineering Osaka University
-
Ishihara Yoshiyuki
Electrical Engineering Doshisya University
-
Shioya S
Department Of Biotechnology Graduate School Of Engineering Osaka University
-
Shioya Suteaki
Department Of Biotechnology Graduate School Of Engineering Osaka University:(present Office)departme
-
Shioya Suteaki
Department Of Biotechnology Faculty Of Engineering Osaka University
-
Shioya Suteaki
Department Of Fermentation Technology Osaka University
-
Shimizu H
Osaka Univ. Osaka Jpn
-
Ishihara Y
Osaka Univ. Osaka Jpn
-
Ishihara Yoshiyuki
Department Of Adaptive Machines Systems Graduate School Of Engineering Osaka University
-
Shimizu Hiroshi
Department Of Animal Science Faculty Of Agriculture Hokkaido University
-
SHIMIZU HIROSHI
Department of Anatomy, School of Medicine, Tokushima University
関連論文
- Differential importance of trehalose accumulation in Saccharomyces cerevisiae in response to various environmental stresses(BIOCHEMICAL ENGINEERING)
- Effect of Sulfur Sources on Specific Desulfurization Activity of Rhodococcus erythropolis KA2-5-1 in Exponential Fed-Batch Culture(Biochemical Engineering)
- PJ-474 Detection of Coronary Atherosclerotic Plaques by Multi-detector Row Computed Tomography : Comparison to Integrated Backscatter Intravascular Ultrasound(CT/DSA-10, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- Animation as a useful tool for assessing functional status in psoriatic arthritis
- ITGB4 missense mutation in a transmembrane domain causes non-lethal variant of junctional epidermolysis bullosa with pyloric atresia
- A novel OSMR mutation in familial primary localized cutaneous amyloidosis in a Japanese family
- Genome-wide expression analysis of Saccharomyces pastorianus orthologous genes using oligonucleotide microarrays(BIOINFORMATICS)
- MN-P20 A model-based analysis method for detection of single-base substitution using resequencing microarrays(Section X Micro/Nano Technology for Analysis and Cell Manipulation)
- FRS-174 Long-term Prognosis of Percutaneous Intervention for Unprotected Left Main Coronary Disease : Comparizon with CABG(Percutaneous Coronary Intervention (IHD) : FRS21)(Featured Research Session (English))
- Comparison of 2D- and 3D- intravascular ultrasound (IVUS) assessments of neointimal hyperplasia of in-stent restenosis
- Relation between Pre-Interventional Neointimal Hyperplasia Burden of In-Stent Restenosis and Chronic Amount of Neointimal Proliferation : A Volumetric Intravascular Ultrasound Study
- Influence of Smoking on Angiographic Restenosis after Percutaneous Coronary Intervention
- The Efficacy of IVUS Guided Stenting for Long Lesion
- Results of the stent versus directional coronary Atherectomy for proximal left anterior descending artery
- Provisional stenting following percutaneous thrombectomy for patients with acute myocardial infarction
- Cardiac Wall Motion Abnormalities Observed in a Patient With Transient Hyperthyroidism
- COL7A1 mutation G2037E causes epidermal retention of type VII collagen
- PJ-202 Coronary Artery Flow Has a Cooling Effect in a Coronary Artery during Radiofrequency Catheter Ablation(Arrhythmia, Non-Pharmacological Therapy 7 (A) : PJ34)(Poster Session (Japanese))
- Kinetic Study of Poly-D(-)-3-Hydroxybutyric Acid (PHB) Production and Its Molecular Weight Distribution Control in a Fed-Batch Culture of Alcaligenes eutrophus
- 572 EFFECT OF CYSTEINE ADDITION ON GLUTATHIONE FERMENTATION BY ___- ___-
- Beta-Carboline Alkaloids in Crude Drugs
- Correlation of clinical severity and ELISA indices for the NC16A domain of BP180 measured using BP180 ELISA kit in bullous pemphigoid
- In vivo transfer of TGF-α and β genes to keratinocytes
- High expression of Ki-67 and cyclin D1 in invasive extramammary Paget's disease
- Low-grade myxofibrosarcoma invaded into the underlying skeletal muscle
- Remodeling of desmosomal and hemidesmosomal adhesion systems during human hair follicle development
- Higher density of label-retaining cells in gingival epithelium
- The surfactant components modulate the immune responses to lipopolysaccharides
- Macrophage migration inhibitory factor (MIF) in bullous pemphigoid
- Interleukin-1β and macrophage migration inhibitory factor (MIF) in dermal fibroblasts mediate UVA-induced matrix metalloproteinase-1 expression
- Effect of CO_2 concentration on the growth and exopolysaccharide production of Bifidobacterium longum cultivated under anaerobic conditions(BIOCHEMICAL ENGINEERING)
- Biological Detoxification of Waste House Wood Hydrolysate Using Ureibacillus thermosphaericus for Bioethanol Production(BIOCHEMICAL ENGINEERING)
- Rational Screening of Antibodies and Design of Sandwich Enzyme Linked Immunosorbant Assay on the Basis of a Kinetic Model(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Strategies for Reducing Supplemental Medium Cost in Bioethanol Production from Waste House Wood Hydrolysate by Ethanologenic Escherichia coli : Inoculum Size Increase and Coculture with Saccharomyces cerevisiae(BIOCHEMICAL ENGINEERING)
- Fed-Batch Coculture of Lactobacillus kefiranofaciens with Saccharomyces cerevisiae for Effective Production of Kefiran(BIOCHEMICAL ENGINEERING)
- Simulation Model for Predicting Limit of Detection and Range of Quantitation of Competitive Enzyme-Linked Immunosorbent Assay(BIOCHEMICAL ENGINEERING)
- Microaeration Enhances Productivity of Bioethanol from Hydrolysate of Waste House Wood Using Ethanologenic Escherichia coli KO11(ENVIRONMENTAL BIOTECHNOLOGY)
- Validation of a Method for Predicting the Precision, Limit of Detection and Range of Quantitation in Competitive ELISA
- Enhancement of Ethanol Production by Promoting Surface Contact between Starch Granules and Arming Yeast in Direct Ethanol Fermentation(BIOCHEMICAL ENGINEERING)
- Optimal Aerobic Cultivation Method for 1,4-Dihydroxy-2-Naphthoic Acid Production by Propionibacterium freudenreichii ET-3(BIOCHEMICAL ENGINEERING)
- Aerobic Culture of Propionibacterium freudenreichii ET-3 Can Increase Production Ratio of 1,4-Dihydroxy-2-Naphthoic Acid to Menaquinone (BIOCHEMICAL ENGINEERING)
- New Strategy for Enhancement of Microbial Viability in Simulated Gastric Conditions Based on Display of Starch-Binding Domain on Cell Surface(BIOCHEMICAL ENGINEERING)
- Expression of C-terminal Repeat Region of Peptidoglycan Hydrolase of Lactococcus lactis IL1403 in Methylotrophic Yeast Pichia pastoris(BIOCHEMICAL ENGINEERING)
- Bidirectional Cell-Surface Anchoring Function of C-Terminal Repeat Region of Peptidoglycan Hydrolase of Lactococcus lactis IL1403(BIOCHEMICAL ENGINEERING)
- Changes in Microflora Duding Composting of an Aquatic Plant, Brazilian Elodea
- A novel P gene missense mutation in a Japanese patient with oculocutaneous albinism type II (OCA2)
- Clinical and genetic analysis of Fabry disease : report of six cases including three heterozygous females
- Novel mutation in a fumalate hydratase gene of a Japanese patient with multiple cutaneous and uterine leiomyomatosis
- Genome-Wide Analysis of the Effects of Location and Number of Stress Response Elements on Gene Expression in Saccharovyces cerevisiae(BIOINFORMATICS)
- Distinct Roles of Two Anaplerotic Pathways in Glutamate Production Induced by Biotin Limitation in Corynebacterium glutamicum(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Improvement of L-Lactate Production by CYB2 Gene Disruption in a Recombinant Saccharomyces cerevisiae Strain under Low pH Condition
- Comparison of Transcriptional Responses to Osmotic Stresses Induced by NaCl and Sorbitol Additions in Saccharomyces cerevisiae Using DNA Microarray(BIOINFORMATICS)
- Dietary glucosylceramide improves skin barrier function in hairless mice
- A unique monoclonal antibody 29A stains the cytoplasm of amniotic epithelia and cutaneous basement membrane
- Possible role of endoplasmic reticulum stress in the pathogenesis of Darier's disease
- Tyrosinase gene analysis in Japanese patients with oculocutaneous albinism
- Nonisothermal Crystallization Kinetics of Biodegradable Random Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) and Block One
- Genetic studies of 20 Japanese families of dystrophic epidermolysis bullosa
- The G2028R glycine substitution mutation in COL7A1 leads to marked inter-familiar clinical heterogeneity in dominant dystrophic epidermolysis bullosa
- Occupational factors, smoking habits and tobacco withdrawal symptoms among male Japanese employees
- Pemphigus : from immunofluorescence to molecular biology
- Compound heterozygosity for premature termination codon and glycine substitution mutations in the COL7A1 gene in Korean siblings with a moderately severe phenotype of recessive dystrophic epidermolysis bullosa
- Spontaneous Giant Aneurysm of the Superficial Temporal Artery : Case Report
- Dependence of Crystal Quality on Residual Strain in Strain-Controlled Thin AlN Layer Grown by Metalorganic Vapor Phase Epitaxy
- UHF Bulk-Acoustic-Wave Filters Utilizing Thin ZnO/SiO_2-Diaphragms on Silicon
- Beer Volatile Compounds and Their Application to Low-Malt Beer Fermentation
- Physiological Analysis of Yeast Cells by Flow Cytometry during Serial-Repitching of Low-Malt Beer Fermentation(BREWING AND FOOD TECHNOLOGY)
- Polymorphic sequences of the tyrosinase gene : allele analysis on 16 OCA1 patients in Japan indicate that three polymorphic sequences in the tyrosinase gene promoter could be powerful markers for indirect gene diagnosis
- R278TER and P431L mutations of the tyrosinase gene exist in Japanese patients with tyrosinase-negative oculocutaneous albinism
- Maximizing Yellow Pigment Production in Fed-Batch Culture of Monascus sp.
- COMBINATION OF HYDRAPEPTIDE PROJECT AND HYDRAEST PROJECT FOR SYSTEMATIC IDENTIFICATION OF NOVEL NEUROPEPTIDES(Physiology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- Tensile Strain Introduced in AlN Layer Grown by Metal-Organic Vapor-Phase Epitaxy on (0001) 6H-SiC with (GaN/AlN) Buffer
- Image Analysis of Mycelial Morphology in Virginiamycin Production by Batch Culture of Streptomyces virginiae
- Maximum Histidine Production by Fed-Batch Culture of Brevibacterium flavum
- Optimum Conditions for Histidine Production by Fed-Batch Culture of Brevibacterium flavum
- Dewatering of Extracellular Polymer and Activated Sludges by Thermochemical Treatment
- Properties of Extracellular Polymer Having an Effect on Expression of Activated Sludge
- 418 Characteristics of a Mutant Brevibacterium flavum Producing Histidine
- 417 EFFECT OF AMINO ACIDS ON GLUTATHIONE PRODUCTION BY SACCHAROMYCES CEREVISIAE
- Dewatering Characteristics of Activated Sludges and Effect of Extracellular Polymer
- Analysis of Hemin Effect on Lactate Reduction in Lactococcus lactis(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Precise Metabolic Flux Analysis of Coryneform Bacteria by Gas Chromatography-Mass Spectrometry and Verification by Nuclear Magnetic Resonance(BIOINFORMATICS)
- Robustness of Cascade pH and Dissolved Oxygen Control in Symbiotic Nisin Production Process System of Lactococcus lactis and Kluyveromyces marxianus(BIOCHEMICAL ENGINEERING)
- Crustal evolution history of Korean Peninsula in East Asia : The significance of Nd, Ce isotopic and REE data from the Korean Precambrian gneisses
- Analysis of Enterococcus faecium N15 plasmid and its use in shuttle vector containing the entI gene as a selection marker
- Knowledge-Based Design and Operation of Bioprocess Systems
- Analysis of a Deep U-Tube Aeration System from the Viewpoint of Oxygen Transfer
- PROCUCTION OF 6-AMINOPENICILLANIC ACID IN IMMOBILIZED ENZYME REACTOR WITH ELECTRODIALYSIS
- Cultured keratinocytes from plectin/HD1-deficient epidermolysis bullosa simplex showed altered ability of adhesion to the matrix
- Mole Fraction Control of Poly(3-Hydroxybutyric-Co-3-Hydroxyvaleric Acid in Fed-Batch Culture of Alcaligenes eutrophus
- Identification of the Hemidesmosomal 500 kDa Protein (HD1) as Plectin
- Epidermal triglyceride levels are correlated with severity of ichthyosis in Dorfman-Chanarin syndrome
- Application of XANES for the Determination of Oxidation States of Co and Pb in Natural Ferromanganese Nodules
- Ion microprobe U-Pb zircon geochronology of the Hida gneiss : Finding of the oldest minerals in Japan
- Interpretation of Pathophysiology by Laboratory Data (3) A Consultation Program Based on Clinical Laboratory Data
- 520 Histidine Fermentation by Brevibacterium flavum FERM 1564-II.
- Comparison of Estimation Techniques for a Time-Dependent Parameter in a Metabolic Reaction Model
- Constraint on the geochemical stage causing tetrad effect in kimuraite : Comparative studies on kimuraite and its related rocks, from REE pattern and Nd isotope ratio
- On the Linearity of Heritability and Genetic Correlation for Juvenile Body Weight and Weight Gain in Meat-type Chickens
- Fast Computation Technique of Genetic Algorithm Based on Finite Element Method