External and Internal Diffusion in a Plate-type Immobilized Enzyme Reactor

概要

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The purpose of the present study is to describe the effect of diffusional restriction on the overall rate of immobilized-enzyme reaction in actual reactor operation. A continuous plate-type reactor, in which Streptomyces sp. K.201 cells containing active glucose isomerase were entrapped with polyacrylamide, was utilized in this work. It is postulated that the fluid flowing downward between the gel plates is in laminar flow. Diffusional restriction consists of the internal resistance within the gel and the simultaneous external resistance created by the liquid film outside the gel. A mathematical model was developed and computer simulation of the experiment was performed. The good agreement between experimental data and numerical values indicates that the proposed diffusion model is correct. The film mass transfer coeffcient, k_l, was determined from the experimental correlation proposed by Chilton & Colburn, and it was concluded that this correlation can be conveniently used for the evaluation of k_l in an immobilized-enzyme reactor under laminar flow condition. In a nonlinear reaction, the effectiveness factor depends on the substrate concentration at the gel surface. Based on this model, the interrelation between internal and external resistance was discussed and the influence of diffusional rsistances on conversion was quantitatively demonstrated.
公益社団法人日本生物工学会の論文
1978-04-25