Production of 1-Kestose by Scopulariopsis brevicaulis

概要

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A 1-kestose-producing fungus, strain N-01,was isolated and identified as Scopulariopsis brevicaulis. The optimal conditions for 1-kestose production by strain N-01 were studied and the following condiitons were established : the strain was cultivated in a 500-ml conical flask containing 25ml of medium composed (per liter) of 150g sucrose, 15g yeast extract, 0.6g urea, 1g K_2HPO_4,and 0.3g MgSO_4・7H_2O (pH7.0) at 30℃ for 72h. When the strain was cultivated in a 2.5-l jar fermentor, 95g of 1-kestose was produced with a theoretical yield of 85%. From this culture broth 1-kestose was crystallized and then recrystallized, giving purities of 98.0 and 99.8% with yield of 71.0 and 78.0%, respectively. In particular, the first crystals in the recrystallization gave over 99.9% purity. By using these crystals, the general properties of 1-kestose were determined; most of these properties coincided with the data in the literature, though the melting point range was narrower. Finally a scheme for the large-scale purification of 1-kestose in proposed.
社団法人日本生物工学会の論文
1994-04-25