Purification and Properties of Mutanase from Bacillus circulans
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概要
- 論文の詳細を見る
Bacterial strain HU-M1 was isolated as a mutant-degrading microbe from soil and identified as Bacillus circulans. From an HU-M1 culture supernatant mutanase was purified 260-fold by ammonium sulfate fractionation and DEAE-Toyopearl 650M (twice), Toyopearl HW55-F, and Butyl-Toyopearl 650M column chromatography with an activity recovery of 10%. The M_r of the mutanae is 160,000 (monomer). The optimal pH and temperature for the mutanase are pH 6.9 and 50℃ for a 10-min reaction. The mutanase is stable at up to 40℃ for 60-min and at pH 6.0 to 11.0 at 4℃ for 48 h, and logarithmically inactivated at 54℃ with a half life of 2.6min. Mutan hydrolysates produced by the enzyme had di-, tri-, and tetramer of glucose and the final hydrolysis was 38%.
- 社団法人日本生物工学会の論文
- 1997-06-25
著者
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Takeda Hiroyuki
Department of Biological Science, Graduate School of Science, The University of Tokyo
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Kinoshita Shinichi
Department of Medicine, Division of Diabetes, Metabolism, and Endocrinology, Kobe University Graduat
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Kinoshita Shinichi
Department Of Fermentation Technology Faculty Of Engineering Osaka University
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Kinoshita Shinichi
Department Of Molecular Chemistry Faculty Of Engineering Hokkaido Graduate University
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Kinoshita S
Hokkaido Univ. Hokkaido Jpn
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Takeda Hiroyuki
Zoological Institute Faculty Of Science University Of Tokyo
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Ooi Toshihiko
Department of Applied Biological Chemistry, College of Agriculture, University of Osaka Prefecture
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Takeda H
Department Of Molecular Chemistry Faculty Of Engineering Hokkaido Graduate University
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Ooi Toshihiko
Department Of Applied Biological Chemistry College Of Agriculture University Of Osaka Prefecture
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Ooi Toshihiko
Department Of Molecular Chemistry Faculty Of Engineering Hokkaido Graduate University
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MATSUDA SHINICHI
Department of Molecular Chemistry, Faculty of Engineering, Hokkaido Graduate University
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KAWANAMI YUUSUKE
Department of Molecular Chemistry, Faculty of Engineering, Hokkaido Graduate University
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Kawanami Yuusuke
Department Of Molecular Chemistry Faculty Of Engineering Hokkaido Graduate University
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Takeda Hiroyuki
Department Of Applied Physics Science University Of Tokyo
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Matsuda Shinichi
Department Of Molecular Chemistry Faculty Of Engineering Hokkaido Graduate University
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Kinoshita Shinichi
Department Of Chemical Process Engineering Faculty Of Engineering Hokkaido University
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