Cloning and Sequence Analysis of the Gene (alyII) Coding for an Alginate Lyase of Pseudomonas sp. OS-ALG-9
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概要
- 論文の詳細を見る
Pseudomonas sp. OS-ALG-9 produces several kinds of alginate-degrading enzymes both intra- and extracellularly. As a second alginate lyase of this bacterium, the gene encoding alyII has been cloned in Escherichia coli JM109 by shotgun techniques and then sequenced. The alyII gene has an open reading frame of 2141 bp encoding 713 amino acid residues with a calculated molecular mass of 79,803 Da. The deduced amino acid sequence did not show any extensive similarity with those of other known alginate lyases, however, hydrophobic cluster analysis showed that alyII belonged to class 3 of alginate lyases. The alginate lyase from E. coli harboring the alyII gene showed a single active band, which coincided with one of four major alginate lyases from the crude cell extracts of Pseudomonas sp. OS-ALG-9 on a zymogram.
- 社団法人日本農芸化学会の論文
- 1997-11-23
著者
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Kraiwattanapong Jintana
Applied Biochemistry Department Of Molecular Chemistry Faculty Of Engineering Hokkaido Graduate Univ
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Kinoshita S
Hokkaido Univ. Hokkaido Jpn
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Ooi Toshihiko
Applied Biochemistry Laboratory Division Of Molecular Chemistry Graduate School Of Engineering Hokka
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Ooi Toshihiko
Applied Biochemistry Department Of Molecular Chemistry Faculty Of Engineering Hokkaido Graduate Univ
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KINOSHITA Shinichi
Applied Biochemistry Laboratory, Division of Molecular Chemistry, Graduate School of Engineering, Ho
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KARAIWATTANAPONG Jintana
Applied Biochemistry, Department of Molecular Chemistry, Faculty of Engineering, Hokkaido Graduate U
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Karaiwattanapong Jintana
Applied Biochemistry Department Of Molecular Chemistry Faculty Of Engineering Hokkaido Graduate Univ
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Kinoshita Shinichi
Applied Biochemistry Department Of Molecular Chemistry Faculty Of Engineering Hokkaido Graduate Univ
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