Physical Map of Nylon Oligomer Degradative Plasmid pOAD2 Harbored in Flavobacterium sp. KI72
スポンサーリンク
概要
- 論文の詳細を見る
Sites of restriction endonucleases were mapped on pOAD2, a plasmid harbored in Flavobacterium sp. KI72. The plasmid codes 6-aminohexanoic acid cyclic dimer hydrolase and 6-aminohexanoic acid linear oligomer hydrolase. pOAD2 (molecular weight: 28.8 megadaltons [Mdal]) had 6 HindIII and 5 EcoRI sites, which were located at 0, 8.4, 8.9, 11.1, 19.0 and 25.0 Mdal (for HinIII) and 3.3, 5.4, 20.4, 20.8, 22.6 Mdal (for EcoRI). A mutant which could not grow on 6-aminohexanoic acid cyclic dimer but grew on the linear dimer as the sole carbon and nitrogen source harbored a deletion plasmid pOAD21 derived from pOAD2. By comparing the restriction sites of these two plasmids, the deleted region was localized on which the 6-aminohexanoic acid cyclic dimer hydrolase was coded.
- 社団法人 日本農芸化学会の論文
著者
-
Negoro Seiji
Department Of Biotechnology Faculty Of Engineering Osaka University
-
Okada Hirosuke
Deparment Of Fermentation Technology Osaka University
-
NEGORO SEIJI
Department of Fermentation Technology, Osaka University
関連論文
- Enzyme-linked Immunosorbent Assay for Nonylphenol Using Antibody-Bound Microfluid Filters in Vertical Fluidic Operation(METHODS)
- Mutational Analysis of the Metabolism of 2,6-Naphthalenedisulfonate by Pigmentiphaga sp. NDS-2
- フロック形成菌Pigmentiphaga sp. NDS-1による2,6-ナフタレンジスルホン酸の生物分解
- Characterization of Hybrid Enzymes between 6-Aminohexanoate-Dimer Hydrolase and Its Analogous Protein
- Alteration of Catalytic Function of 6-Aminohexanoate-Dimer Hydrolase by Site-Directed Mutagenesis
- Expression of the Xylan-Degrading Genes of Bacillus pumilus IPO in Saccharomyces cerevisiae
- 1P042 Molecular dynamics studies on Mutational structures of a Nylon-6 Byproduct-degrading Enzyme(Protein:Structure & Function,The 48th Annual Meeting of the Biophysical Society of Japan)
- Construction and Characterization of N-Terminally Truncated DNA Polymerase from Thermus thermophilus
- Operational Stability of a Continuous Enzyme Reactor Containing Poly(ethylene glycol)-Bound NAD and Thermostable Dehydrogenases
- Analysis of a Promoter-Like Region in Flavobacterium Which Controls 6-Aminohexanoic Acid Linear Oligomer Hydrolase Gene Expression in Escherichia coli
- 408 The Role of Asp21 of the Xylanase from Bacillus pumilus IPO
- Purification and Properties of Aminopeptidase from Flavobacterium sp.
- Genetic Organization of Nylon-Oligomer-Degrading Enzymes from Alkalophilic Bacterium, Agromyces sp. KY5R(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Purification and Characterization of Alkylcatechol 2,3-Dioxygenase from Butylphenol Degradation Pathway of Pseudomonas putida MT4(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Characterization of Alkylphenol Degradation Gene Cluster in Pseudomonas putida MT4 and Evidence of Oxidation of Alkylphenols and Alkylcatechols with Medium-Length Alkyl Chain(ENVIRONMENTAL BIOTECHNOLOGY)
- Characterization of Endo-Type 6-Aminohexanoate-Oligomer Hydrolase from Flavobacterium sp.
- Structure of Isozyme Genes of Glucose Dehydrogenase from Bacillus megaterium IAM1030
- Purification and Characterization of a New Glucose Dehydrogenase from Vegetative Cells of Bacillus megaterium
- Reaction of Glutaraldehyde with Amino and Thiol Compounds
- Analysis and Improvement of the Synthesis of N^6-(2-Carboxyethyl)-NAD
- Preparation and Coenzymic Properties of 3-(N-Methyl)Carbamoylpyridine-Adenine Dinucleotide and 3-[N-(2-Aminoetyl)]Carbamoylpyridine-Adenine Dinucleotide
- Tetrazolium Salt Method Coupled with a Phenazinium Salt Gives Higher Rates for Dehydrogenase Reactions
- Simultaneous Degradation of 4-Nitrophenol and Picric Acid by Two Different Mechanisms of Rhodococcus sp. PN1
- Cloning and Characterization of a 4-Nitrophenol Hydroxylase Gene Cluster from Rhodococcus sp. PN1
- Secretive Expression of the Aspergillus aculeatus Cellulase (FI-CMCase) by Saccharomyces cerevisiae
- Expression of the Cellulase (FI-CMCase) Gene of Aspergillus aculeatus in Saccharomyces cerevisiae
- Expression of the Cellulase (FI-CMCase) Gene of Aspergillus aculeatus in Escherichia coli
- A Simple and Rapid Extraction of High Molecular Weight Chromosomal DNA from Bacillus subtilis Protoplasts for Cosmid Cloning and Interspecific Transformation
- Simple Detection of Xylosidase Activity in Single Colonies, Using 1-Naphthyl-β-D-xylopyranoside
- A Simple Assay for Xylanase Using o-Nitrophenyl-β-D-xylobioside
- 枯草菌Marburg株の生産するα-アミラーゼの性質(ノート)〔英文〕
- Biodegradability of 3-Methyl-4-Nitrophenol by 4-Nitrophenol-Degrading Bacteria
- Immunoassay Using Microfluid Filters Constructed by Deep X-Ray Lithography
- 枯草菌Marburg株のα-アミラーゼ生産能の研究-1-高生産株の分離とそれらの酵素の比較〔英文〕
- Purification and Characterization of Catechol 2,3-Dioxygenase from the Aniline Degradation Pathway of Acinetobacter sp. YAA and Its Mutant Enzyme, Which Resists Substrate Inhibition
- Identification and characterization of another 4-nitrophenol degradation gene cluster, nps, in Rhodococcus sp. strain PN1(ENVIRONMENTAL BIOTECHNOLOGY)
- Comparison of Acyl-CoA Synthetic Activities and Enantioselectivity toward 2-Arylpropanoic Acids in Firefly Luciferases
- Properties of Glucose-Dehydrogenase-Poly(Ethylene Glycol)-NAD Conjugate as an NADH-Regeneration Unit in Enzyme Reactors
- Kinetics of α-glucoside fermentation controlled by substrater permeation (Proceedings of the U.S.Japan seminar on dynamics of microbial populations(特集))
- Kinetic Studies on Enzyme Production by Microbes : (II) Process Kinetics of α-Amylase Production by Bacillus subtilis
- Protoplast Formation from Conidia of Trichoderma reesei by Cell Wall-Lytic Enzymes of a Strain of Trichoderma viride
- Comparison of Acyl-CoA Synthetic Activities and Enantioselectivity toward 2-Arylpropanoic Acids in Firefly Luciferases
- Hydration of Squalene and Oleic Acid by Corynebacterium sp. S-401
- Purification and Properties of Endoxylanase Produced by Bacillus pumilus
- Physical Map of Nylon Oligomer Degradative Plasmid pOAD2 Harbored in Flavobacterium sp. KI72
- Asymmetric Synthesis of (S)-(-) Squalene-2, 3-epoxide