High-Throughput and Highly Sensitive Analysis Method for Polyisoprene in Plants by Pyrolysis-Gas Chromatography/Mass Spectrometry

スポンサーリンク

概要

• 論文の詳細を見る

• Natural polyisoprene is a biopolymer consisting of isoprene units (C5H8) that is used commercially in household, medical, and industrial materials. For the management of natural polyisoprene production, the selection of high-yield polyisoprene-producing trees, and an understanding of polyisoprene biosynthesis, a high-throughput and highly sensitive screening method for the quantification of polyisoprene is required. In this study, we examined pyrolysates from polyisoprenes, polyprenols, carotenoids, ubiquinone (CoQ-10), and sterols by pyrolysis gas chromatography/mass spectrometry (PyGC/MS) and determined that the amounts of isoprene and limonene released from polyprenols and polyisoprenes were dependent upon their molecular weights. Based on these results, we developed a relative quantification method for polyisoprene in leaves by direct analysis of 1 mg of leaves using PyGC/MS. This novel quantification method eliminated extraction steps and can be used in the measurement of polyisoprene contents in Eucommia ulmoides and Hevea brasiliensis.

• 社団法人 日本農芸化学会の論文
• 2010-01-23

著者

• Bamba Takeshi

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• Fukusaki Eiichiro

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• NAKAZAWA Yoshihisa

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• FUKUSAKI EI-ICHIRO

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• KOBAYASHI AKIO

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• KAWABE Akira

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• Kobayashi A

  National Inst. Advanced Industrial Sci. And Technol. Ibaraki Jpn

• Bamba Takeshi

  Department Of Applied Environmental Biology Graduate School Of Pharmaceutical Sciences Osaka Univers

• Kobayashi Akio

  Dep. Of Biotechnology Graduate School Of Engineering Osaka Univ.

• Kobayashi A

  Laboratory Of Bioresources Chemistry Faculty Of Agriculture Okayama University

• OKAZAWA Atsushi

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• Kobayashi A

  Ochanomizu Univ. Tokyo Jpn

• Kobayashi A

  Dep. Of Biotechnology Graduate School Of Engineering Osaka Univ.

• Kawabe Akira

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Bamba Takeshi

  Dep. Of Biotechnology Graduate School Of Engineering Osaka Univ. 2- 1 Yamadaoka Suita Osaka 565-0871

• Bamba Takeshi

  Dept. Biotech. Grad. Sch. Eng. Osaka Univ.:hitachi Zosen Co.

• TAKENO Shinya

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• Nakazawa Yoshihisa

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Fukusaki Eiichiro

  Division Of Advanced Science And Biotechnology Graduate School Of Engineering Osaka University

• Fukusaki Eiichiro

  Dep. Of Biotechnology Graduate School Of Engineering Osaka Univ.

• Fukusaki Ei-ichiro

  大阪大学大学院

• Fukusaki E

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Fukusaki Eiichiro

  Department Of Biotechnology Faculty Of Engineering Osaka University

• Fukusaki Eiichiro

  Dept. Mat. Life Sci. Osaka Univ

• Okazawa Atsushi

  Dep. Of Biotechnology Graduate School Of Engineering Osaka Univ.

• Okazawa Atsushi

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Okazawa Atsushi

  Department Of Applied Physics And Chemistry The University Of Electro-communications

• Takeno Shinya

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Kobayashi Akio

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• KOBAYASHI Akio

  Department of Biochemistry, Shinshu University School of Medicine

• KOBAYASHI Akio

  Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku University

関連論文

• Pyrolysis GC-MS-based metabolite fingerprinting for quality evaluation of commercial Angelica acutiloba roots(METHODS)
• Size Exclusion Chromatography of Standard Polystyrenes with a Wide Range of Molecular Weight Up to 7.45 × 10^6 on Monolithic Silica Capillary Columns
• Production of Eucommia-rubber from Eucommia ulmoides Oliv. (Hardy Rubber Tree)
• DrEFTIR : The data mining software for fourier transform near-infrared reflectance spectroscopy focused on food metabolic finger printing
• Characterization of the C-terminal truncated form of amylopullulanase from Lactobacillus plantarum L137(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Establishment of an Agrobacterium-mediated transformation system for Periploca sepium Bunge
• An approach to peak detection in GC-MS chromatograms and application of KNApSAcK database in prediction of candidate metabolites
• Influences of biofluid sample collection and handling procedures on GC-MS based metabolomic studies(METHODS)
• Establishment of an Agrobacterium-mediated transformation system for Periploca sepium Bunge
• Characterization of Gene Encoding Amylopullulanase from Plant-Originated Lactic Acid Bacterium, Lactobacillus plantarum L137(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Introduction of the Archaebacterial Geranylgeranyl Pyrophosphate Synthase Gene into Chlamydomonas reinhardtii Chloroplast
• Application of the Bio-Active Beads Method in Rice Transformation
• An Arabidopsis thaliana Gene on the Yeast Artificial Chromosome Can Be Transcribed in Tobacco Cells
• Removal of Phenolic Endocrine Disrupters by Portulaca oleracea(ENVIRONMENTAL BIOTECHNOLOGY)
• Biosynthetic Pathway for the C45 Polyprenol, Solanesol, in Tobacco
• Transient RNA Silencing of Scoulerine 9-O-Methyltransferase Expression by Double Stranded RNA in Coptis japonica Protoplasts
• Isolation and Characterization of a cDNA Encoding Phytochrome A in the Non-Photosynthetic Parasitic Plant, Orobanche minor Sm
• Comparative analysis of a CFo ATP synthase subunit II homologue derived from marine and fresh-water algae(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
• Isolation and characterization of a novel antistress gene from Chlamydomonas sp. W80(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
• Transient Gene Expression in Guard Cell Chloroplasts of Tobacco Using ArF Excimer Laser Microablation(PLANT BIOTECHNOLOGY)
• Synthesis of Anti-Tumor Dimeric Indole Alkaloids in Catharanthus roseus Was Promoted by Irradiation with Near-Ultraviolet Light at Low Temperature
• A Novel Gene Delivery System in Plants with Calcium Alginate Micro-Beads(PLANT BIOTECHNOLOGY)
• Isolation and Characterization of Two Novel Nematicidal Depsipeptides from an Imperfect Fungus, Strain D1084
• 1Mp19 Unique peptides were produced in in vitro tryptic digestion of α-casein in the presence of a phenolic compound, gallic acid
• 2Gp12 Application of supercritical fluid chromatography/mass spectrometry (SFC/MS) to lipid profiling of soybean
• Functional Analysis of Two Solanesyl Diphosphate Synthases from Arabidopsis thaliana
• Transient RNAi Induction against Endogenous Genes in Arabidopsis Protoplasts Using in Vitro-Prepared Double-Stranded RNA
• A Transient RNA Interference Assay System Using Arabidopsis Protoplasts(Biochemistry & Molecular Biology)
• Expression and Purification of Recombinant Human Annexin A2 in Pichia pastoris and Utility of Expression Product for Detecting Annexin A2 Antibody(MEDICAL BIOTECHNOLOGY)
• 3B11-1 A Rapid Comparison of Large-scale GC-MS Data for Metabolomics.
• 3B11-3 Metabolic Profiling of Camellia sinensis (Tea) Using GC/MS
• Overexpression of 1-Deoxy-D-Xylulose-5-Phosphate Reductoisomerase Gene in Chloroplast Contributes to Increment of Isoprenoid Production(PLANT BIOTECHNOLOGY)
• Obtaining transgenic plants using the bio-active beads method
• Guidelines for the treatment of Crohn's disease in children
• A New Organic Superconductor, λ-(BEDT-TSF)_2GaCl_4
• Molecular and functional characterization of novel furofuran-class lignan glucosyltransferases from Forsythia
• Molecular and functional characterization of novel furofuran-class lignan glucosyltransferases from Forsythia
• Comprehensive metabolite profiling of phyA phyB phyC triple mutants to reveal their associated metabolic phenotype in rice leaves(PLANT BIOTECHNOLOGY)
• High Throughput and Exhaustive Analysis of Diverse Lipids by Using Supercritical Fluid Chromatography-Mass Spectrometry for Metabolomics(METHODS)
• Quantitative Analysis of Anionic Metabolites for Cathamnthus roseus by Capillary Electrophoresis Using Sulfonated Capillary Coupled with Electrospray lonization-Tandem Mass Spectrometry(METHODS)
• Single Cell-Based Analysis of Torenia Petal Pigments by a Combination of ArF Excimer Laser Micro Sampling and Nano-High Performance Liquid Chromatography (HPLC)-Mass Spectrometry(METHODS)
• Pressure-Assisted Capillary Electrophoresis Mass Spectrometry Using Combination of Polarity Reversion and Electroosmotic Flow for Metabolomics Anion Analysis(METHODS)
• Stable Isotope Dilution-Based Accurate Comparative Quantification of Nitrogen-Containing Metabolites in Arabidopsis thaliana T87 Cells Using in Vivo ^N-Isotope Enrichment
• An Isotope Effect on the Comparative Quantification of Flavonoids by Means of Methylation-Based Stable Isotope Dilution Coupled with Capillary Liquid Chromatography/Mass Spectrometry(Methods)
• Two-Dimensional Quantitative Analysis of Preferential Alignment of BAp c-axis for Isolated Human Trabecular Bone Using Microbeam X-ray Diffractometer with a Transmission Optical System
• Novel plant transformation system by gene-coated gold particle introduction into specific cell using ArF excimer laser
• Oxazolomycin Esters, Specific Inhibitors of Plant Transformation
• Plant Metabolomics : Potential for Practical Operation
• A Novel Bioassay Method for Inhibitors of Plant Transformation
• Production of a New Type of Bioactive Phenolic Compound
• Bisanthraquinones, Inhibitors of Plant Transformation
• ST-1, a Novel Radical Scavenger From Fungus F-124
• Quantitation of formate by solid-phase microextraction and gas chromatography-mass spectrometry utilizing a [^C]formate internal standard
• Methylamine Treatment Changes the Allocation of Carbohydrate to Roots in Rice Plants(ENVIRONMENTAL BIOTECHNOLOGY)
• Formate Dehydrogenase Gene of Arabidopsis thaliana is Induced by Formaldehyde and Not by Formic Acid
• Formate Protects Photosynthetic Machinery from Photoinhibition
• Formate Dehydrogenase in Rice Plant : Growth Stimulation Effect of Formate in Rice Plant
• An Artificial Plastic Receptor That Discriminates Axial Asymmetry
• New Antimicrobial Compound from Scorodocarpus borneensis Becc.
• Purification and Properties of Human D-3-Hydroxyacyl-CoA Dehydratase: Medium-Chain Enoyl-CoA Hydratase Is D-3-Hydroxyacyl-CoA Dehydratase
• Two Mitochondrial 3-Hydroxyacyl-CoA Dehydrogenases in Bovine Liver^1
• Lipase-Catalyzed Kinetic Resolution of 2, 3-Epoxy-1-Tridecanol and Its Application to Facile Synthesis of (+)-Disparlure
• Lipase-Catalyzed Kinetic Resolution of 4-Hydroxy-5-Dodecynonitrile and Its Application to Facile Synthesis of a Cupreous Chafer Beetle Sex Pheromone
• Synthesis of the Four Possible Stereoisomers of 21-Methyl-8-Pentatriacontene, the Female Contact Sex Pheromone of the Yellow-Spotted Longicorn Beetle, Psacothea hilaris
• Sex Pheromonal Activity of Geometric and Optical Isomers of Synthetic Contact Pheromone to Males of the Yellow-Spotted Longicorn Beetle, Psacothea hilaris (PASCOE) (Coleoptera: Cerambycidae)
• Synthesis of the Enantiomers of (Z)-5(1-Octenyl)oxacyclopentan-2-one, a Sex Pheromone of the Cupreous Chafer Beetle, Anomala cuprea Hope
• Large-Scale Preparation of (+)-Disparlure, the Gypsy Moth Pheromone, by a Practical Chemico-Enzymatic Procedure
• Lipase-Catalyzed Kinetic Resolution of 2,3-Epoxy-8-Methyl-1-Nonanol, the Key Intermediate in the Synthesis of the Gypsy Moth Pheromone
• A High-Throughput and Solvent-free Method for Measurement of Natural Polyisoprene Content in Leaves by Fourier Transform Near Infrared Spectroscopy(METHODS)
• Metabolic Profiling of Angelica acutiloba Roots Utilizing Gas Chromatography-Time-of-Flight-Mass Spectrometry for Quality Assessment Based on Cultivation Area and Cultivar via Multivariate Pattern Recognition(BREWING AND FOOD TECHNOLOGY)
• Quantification of trans-1,4-Polyisoprene in Eucommia ulmoides by Fourier Transform Infrared Spectroscopy and Pyrolysis-Gas Chromatography/Mass Spectrometry(METHODS)
• 1J10-4 ^1H NMR BASED METABOLIC PROFILING IN THE QUALITY EVALUATION OF JAPANESE GREEN TEA
• Phytochelatins inhibit the metal-induced aggregation of α-crystallin(PLANT BIOTECHNOLOGY)
• Effect of simvastatin on steroid-induced osteonecrosis evidenced by the serum lipid level and hepatic cytochrome P4503A in a rabbit model
• Aroma Compounds in the Leaves of Japanese Pepper (Zanthoxylum piperium DC) and Their Formation from Glycosides
• Identification and Antimicrobial Activity of the Volatile Flavor Constituents from Scorodocarpus borneensis Becc
• A β-Hydroxychalcone and Flavonoids from Alfalfa Callus Stimulated by a Fungal Naphthoquinone, PO-1(Organic Chemistry)
• Unique Spindle Poisons, Curvularin and Its Derivatives, Isolated from Penicillium Species(Organic Chemistry)
• Metabolic Engineering of Lignan Biosynthesis in Forsythia Cell Culture
• Effects of Dietary Nucleotide Supplementation on the Plasma Lipoprotein, Fatty Acids and Uric Acid in Early Infancy
• Effect of Dietary Nucleotide Supplementation on Development of the Faecal Flora in Newborn Infants
• Reaction of Copper Oxide and β-Diketone for In situ Cleaning of Metal Copper in a Copper Chemical Vapor Deposition Reactor
• Deposition Rate and Gap Filling Characteristics in Cu Chemical Vapor Deposition with Trimethylvinylsilyl Hexafluoro-acetylacetonate Copper (I)
• Effects of light on production of endogenous and exogenous lignans by Forsythia koreana wildtype and transgenic cells
• Synthesis of Anti-Tumor Dimeric Indole Alkaloids in Catharanthus roseus Was Promoted by Irradiation with Near-Ultraviolet Light at Low Temperature
• Cloning and Characterization of Mevalonate Pathway Genes in a Natural Rubber Producing Plant, Hevea brasiliensis
• High-Throughput and Highly Sensitive Analysis Method for Polyisoprene in Plants by Pyrolysis-Gas Chromatography/Mass Spectrometry
• Cloning and Characterization of the 2-C-Methyl-D-erythritol 4-Phosphate (MEP) Pathway Genes of a Natural-Rubber Producing Plant, Hevea brasiliensis
• Molecular Cloning and Partial Characterization of a Peroxidase Gene Expressed in the Roots of Portulaca oleracea cv., One Potentially Useful in the Remediation of Phenolic Pollutants
• Application of Fourier transform near-infrared spectroscopy to optimization of green tea steaming process conditions(BREWING AND FOOD TECHNOLOGY)
• Simultaneous quantification of lignans in Arabidopsis thaliana by highly sensitive capillary liquid chromatography-electrospray ionization-ion trap mass spectrometry
• Application of supercritical fluid chromatography/mass spectrometry to lipid profiling of soybean(METHODS)
• Application of electrospray ionization ion trap/time-of-flight mass spectrometry for chemically-synthesized small RNAs(METHODS)
• Metabolic profiling and identification of the genetic varieties and agricultural origin of Cnidium officinale and Ligusticum chuanxiong(PLANT BIOTECHNOLOGY)
• Highly sensitive and rapid profiling method for carotenoids and their epoxidized products using supercritical fluid chromatography coupled with electrospray ionization-triple quadrupole mass spectrometry(METHODS)
• Gas chromatography-mass spectrometry based metabolic profiling for the identification of discrimination markers of Angelicae Radix and its application to gas chromatography-flame ionization detector system(METHODS)
• Gallic acid oxidizes Met residues in peptides released from bovine β-lactoglobulin by in vitro digestion(BREWING AND FOOD TECHNOLOGY)
• Highly sensitive and rapid profiling method for carotenoids and their epoxidized products using supercritical fluid chromatography coupled with electrospray ionization-triple quadrupole mass spectrometry
• Effect of gallic acid on peptides released by trypsin digestion of bovine α-casein(BREWING AND FOOD TECHNOLOGY)
• 3Ap08 Application of Metabolomics to high resolution phenotype analysis(Japan-Korea Biomass Session)

もっと見る 閉じる

スポンサーリンク