Molecular Cloning and Partial Characterization of a Peroxidase Gene Expressed in the Roots of Portulaca oleracea cv., One Potentially Useful in the Remediation of Phenolic Pollutants
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概要
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Portulaca (Portulaca oleracea cv.) efficiently removes phenolic pollutants from hydroponic solution. In plant roots, peroxidase (PRX) is thought to be involved in the removal of phenolic pollutants by the cross-linking them to cell wall polysaccharides or proteins at the expense of reduction of hydrogen peroxide (H2O2). In this study, we found that portulaca roots secreted an acidic PRX isozyme that had relatively high H2O2 affinity. We isolated five PRX genes, and the recombinant PRX proteins produced in cultured tobacco cells were partially characterized. Among these genes, PoPRX2 probably encoded the acidic PRX isozyme. PoPRX2 had an extra N-terminal region which has not been reported for other PRX proteins. We found that PoPRX2 oxidized phenolic pollutants, including bisphenol A, octylphenol, nonylphenol, and 17β-estradiol. In addition, we found that the Cys261 residue of PoPRX2 played an important role in the determination of affinity for H2O2 and stability toward H2O2.
- 社団法人 日本農芸化学会の論文
著者
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Bamba Takeshi
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Hirata Kazumasa
Applied Environmental Biology Graduate School Of Pharmaceutical Sciences Osaka University
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Matsui Takeshi
Graduate School of Biological Sciences, Nara Institute of Science and Technology
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Nakayama Hideki
Graduate School of Biological Sciences, Nara Institute of Science and Technology
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Kato Ko
Graduate School of Biological Sciences, Nara Institute of Science and Technology
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MIYASAKA HITOSHI
Kansai Electric Power Co., Technical Research Center
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Harada Kazuo
Applied Environmental Biology Graduate School Of Pharmaceutical Sciences Osaka University
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Takagi Masahiro
School Of Material Science Japan Advanced Institute Of Science And Technology
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NOMURA Yuki
Graduate School of Biological Sciences, Nara Institute of Science and Technology (NAIST)
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TAKANO Mai
Graduate School of Biological Sciences, Nara Institute of Science and Technology (NAIST)
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IMAI Sofue
Applied Environmental Biology, Graduate School of Pharmaceutical Sciences, Osaka University
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OKUHATA Hiroshi
Kansai Electric Power Co., Inc., Environmental Research Center
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TANAKA Satoshi
Kansai Electric Power Co., Inc., Environmental Research Center
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MATSUURA Hideyuki
Applied Environmental Biology, Graduate School of Pharmaceutical Sciences, Osaka University
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