Strong Induction of Phytochelatin Synthesis by Zinc in Marine Green Alga, Dunaliella tertiolecta

スポンサーリンク

概要

• 論文の詳細を見る

• Synthesis of phytochelatins(PCs), heavy-metal-sequestering peptides, in the marine green alga, Dunaliella tertiolecta, was evaluated under various conditions of exposure to heavy metals. To investigate the effect of heavy metals on both PC synthesis and their upstream biosynthetic reactions, an ion-pair-HPLC system was developed in this study, by which PCs and their biosynthetic intermediates, cysteine(Cys), γ-glutamylcysteine(γEC)and glutathione(GSH), could be determined simultaneously with high sensitivity. When the cells were exposed to Zn^<2+> the level of PCs was maximal at 200 μM and significantly higher than that obtained after exposure to 400 μM Cd^<2+> which is the strongest inducer of PC synthesis in higher plants in vivo and in vitro as well as in microalgae. The predominant PC subtype was PC_4, followed by PC_3 and PC_5, whereas PC_2, which is generally abundant in higher plants, has the lowest level among PC_2 to PC_5. These results suggest that the characteristics of PC synthase in D.tertiolecta including the requirement of heavy metals for its catalysis and substrate specificity towards GSH and PC_n are considerably different from those is higher plants and other algae. While PC synthese proceeded in the heavy-metal-treated cells, the level of GSH did not appreciably change. To maintain the same size of the GSH pool, GSH must be newly synthesized to balance the amount consumed for PC synthesis.

• 社団法人日本生物工学会の論文
• 2001-07-25

著者

• Zenk Meinhart

  Biozentrum-pharmazie Universitat Halle

• Ohta Toshiko

  Environmental Bioengineering Laboratory Graduate School Of Pharmaceutical Sciences Osaka University

• HIRATA KAZUMASA

  Environmental Bioengineering Laboratory, Graduate School of Pharmaceutical Sciences, Osaka Universit

• TSUJIMOTO YOSHIMASA

  Environmental Bioengineering Laboratory, Graduate School of Pharmaceutical Sciences, Osaka Universit

• NAMBA TAKASHI

  Environmental Bioengineering Laboratory, Graduate School of Pharmaceutical Sciences, Osaka Universit

• HIRAYANAGI NAYUMI

  Environmental Bioengineering Laboratory, Graduate School of Pharmaceutical Sciences, Osaka Universit

• MIYASAKA HITOSHI

  Kansai Electric Power Co., Technical Research Center

• MIYAMOTO KAZUHISA

  Environmental Bioengineering Laboratory, Graduate School of Pharmaceutical Sciences, Osaka Universit

• Hirata Kazumasa

  Environmental Bioengineering Laboratory Graduate School Of Pharmaceutical Sciences Osaka University

• Hirata Kazumasa

  Environ. Bioeng. Lab Grad. Sch. Pharm. Sci. Osaka Univ.

• Miyamoto Kazuhisa

  Environmental Bioengineering Laboratory Graduate School Of Pharmaceutical Sciences Osaka University

• Miyasaka Hitoshi

  Kansai Electric Power Co. Inc.

• Namba Takashi

  Environmental Bioengineering Laboratory Graduate School Of Pharmaceutical Sciences Osaka University

• Hirayanagi Nayumi

  Environmental Bioengineering Laboratory Graduate School Of Pharmaceutical Sciences Osaka University

• Tsujimoto Y

  Environmental Bioengineering Laboratory Graduate School Of Pharmaceutical Sciences Osaka University

• Miyasaka Hitoshi

  Kansai Electric Power Co. Technical Research Center

• Miyamoto Kazuhisa

  Environmental Bioengineering Laboratory Faculty Of Pharmaceutical Sciences Osaka University

• Miyasaka Hitoshi

  Kansai Electric Co. Environmental Research Center

• Hirota Kazumasa

  Environmental Bioengineering Laboratory, Graduate School of Pharmaceutical Sciences, Osaka University

関連論文

• Strong Induction of Phytochelatin Synthesis by Zinc in Marine Green Alga, Dunaliella tertiolecta
• Removal of Hazardous Phenols by Microalgae under Photoautotrophic Conditions
• Magnetic-Field-Induced Freezing of Spin Correlations in Networked System of [Mn_4] Single-Molecule Magnets(Condensed matter: electronic structure and electrical, magnetic, and optical properties)
• Two-Dimensional Coordination Networks of [Mn_4] Single-Molecule Magnets : Heat Capacity Measurements(Condensed matter : structure and mechanical and thermal properties)
• Systematic Synthesis of Porphyrin Dimers Linked by Conjugated Oligoacetylene Bridges
• Pyrene-Fused Porphyrins : Annulation Reactions of meso-Pyrenylporphyrins
• Relationships between length and weight of freshwater macroinvertebrates in Japan
• Length–weight Relationships in Six Amphibian Species of Japan
• A seasonal change in the distribution of a stream-dwelling stonefly nymph reflects oxygen supply and water flow
• Push-up response of stonefly larvae in low-oxygen conditions
• Sand banks contribute to the production of coastal waters by making a habitat for benthic microalgae in the sublittoral zone : food web analyses in Aki-Nada using stable isotopes
• A Square Cyclic Porphyrin Dodecamer : Synthesis and Single-Molecule Characterization
• Tuning of Electronic Structures of Quasi-One-Dimensional Bromo-Bridged Pd^-Pd^ Mixed-Valence Complexes by Substituting Counter Anions
• Versatile Vapochromic Behavior Accompanied by a Phase Change between Charge-Polarization State and Charge-Density-Wave State in a Quasi-One-Dimensional Iodo-Bridged Dinuclear Platinum Mixed-Valence Compound, [{NH_3(CH_2)_5NH_3}_2]-[Pt_2(pop)_4I]・4H_2O
• Metal-Metal Bonded Diruthenium Unit Axial-Capped by Di-tert-butylphenolate : [Ru_2(O_2CCH_3)_2(t-Busal-R'py)_2]^- (t-Busal-R'py^ = N-(R'-2-pyridyl)-2-oxido-3,5-di-tert-butylbenzylaminato ; R' = H, 4-Me, and 5-Me)
• New Charge-transfer Salts Based on Unsymmetrical Donor DMET and Metal Complex Anions : (DMET)_3[Cr(isoq)_2(NCS)_4] and (DMET)_3[Cr(phen)(NCS)_4]・CH_3CN
• Synthesis and Structural Determination of a Porphyrinatoplatinum(II) : meso-Tetrakis(4-t-butylphenyl)porphyrinatoplatinum(II)
• Unprecedented Soliton Formation Mechanism in Quasi-One-Dimensional Chloro-Bridged Pt^-Pt^ Mixed-Valence Compound, {[Pt(en)_2][PtCl_2(en)_2]}_3(CuCl_4)_4・12H_2O
• 23pWX-6 Scanning Tunneling Microscopy Investigation of tris(phthalocyaninato)yttrium triple-decker molecules deposited on Au(111)
• Excretion of Glycerol by the Marine Chlamydomonas sp.Strain W-80 in High CO_2 Cultures
• Enhancement of Starch Degradation by CO_2 in a Marine Green Alga, Chlamydomonas sp. MGA161
• Rapid Method for Detection and Detoxification of Heavy Metal Ions in Water Environments Using Phytochelatin
• Characteristics of Biological NOx Removal from Flue Gas in a Dunaliella tertiolecta Culture System
• Improvement of Selective Removal of Heavy Metals in Cyanobacteria by NaOH Treatment(Environmental Biotechnology)
• Degradation of Carbendazim and 2, 4-Dichlorophenoxyacetic Acid by Immobilized Consortium on Loofa Sponge(ENVIRONMENTAL BIOTECHNOLOGY)
• Effect of Algal Extract on H_2 Production by a Photosynthetic Bacterium Rhodobium marinum A-501 : Analysis of Stimulating Effect Using a Kinetic Model(ENVIRONMENTAL BIOTECHNOLOGY)
• Improvement of Microalgal NOx Removal in Bubble Column and Airlift Reactors
• Selective Cadmium Removal from Hard Water Using NaOH-Treated Cells of the Cyanobacterium Tolypothrix tenuis
• Removal of Cadmium from Aqueous Solution by the Filamentous Cyanobacterium Tolypothrix tenuis
• Biological Elimination of Nitric Oxide and Carbon Dioxide from Flue Gas by Marine Microalga NOA-113 Cultivated in a Long Tubular Photobioreactor
• Role of phytoplankton size distribution in lake ecosystems revealed by a comparison of whole plankton community structure between Lake Baikal and Lake Biwa
• Photoproduction of Hydrogen from Raw Starch Using a Halophilic Bacterial Community
• Hydrogen Photoproduction from CO_2-Fixing Microalgal Biomass : Application of Halotolerant Photosynthetic Bacteria
• Flow rate mediates the competitive influence of a grazing caddisfly on mayflies
• Biosynthetic Regulation of Phytochelatins, Heavy Metal-Binding Peptides
• Bioactivities of Nostocine A Produced by a Freshwater Cyanobacterium Nostoc spongiaeforme TISTR 8169
• H_2 Production from Algal Biomass by a Mixed Culture of Rhodobium marinum A-501 and Lactobacillus amylovorus
• Catharanthine Oxidation in Flavin Mononucleotide-Mediated Catharanthine-Vindoline Coupling Reaction for Synthesis of Dimeric Indole Alkaloids under Near-Ultraviolet Light
• Non-Enzymic Coupling of Vindoline and Catharanthine to Synthesize 3', 4'-Anhydrovinblastine under Irradiation with Near-Ultraviolet Light
• Seasonal dynamics of primary production in the pelagic zone of southern Lake Baikal
• Syntheses, Crystal Structure, and Magnetic Properties of Mn_ Single-Molecule Magnets with Naphthalenecarboxylate Bridges, [Mn_O_(O_2CC_H_7)_(H_2O)_4] and Their Tetraphenyl-phosphonium Salts
• 910 Enhance accumulation of Cd^ by Rhizobium expressing phytochelatin synthase
• Stereoselective Conversion of Anhydrovinblastine into Vinblastine Utilizing an Anti-vinblastine Monoclonal Antibody as a Chiral Mould
• Metal-Metal Bonded Diruthenium Unit Axial-Capped by Di-tert-butylphenolate: [Ru2(O2CCH3)2(t-Busal-R'py)2]〔-〕 (t-Busal-R'py2〔-〕= N-(R'-2-pyridyl)-2-oxido-3,5-di-tert-butylbenzylaminato; R'= H, 4-Me, and 5-Me)
• Construction of a One-Dimensional Chain Composed of Mn_6 Clusters and 4,4'-Bipyridine Linkers: The First Step for Creation of "Nono-Dots-Wires"
• A Novel Mn_ Single-molecule Magnet with a μ_3-Methanesulfonate Bridge
• A Chemical Modification of a Mn_ Single-Molecule Magnet by Replacing Carboxylate Anions with Diphenylphosphate Anions
• Alterations in the grazing activities of cased caddisfly larvae in response to variations in predation risk and resource level
• Thermal changes in the gastric evacuation rate of the freshwater sculpin Cottus nozawae Snyder
• Selective foraging on terrestrial invertebrates by rainbow trout in a forested headwater stream in northern Japan
• The rbcX Gene Product Promotes the Production and Assembly of Ribulose-1, 5-Bisphosphate Carboxylase/Oxygenase of Synechococcus sp. PCC7002 in Escherichia coli
• Co_2 Response Element and Corresponding trans-acting Factor of the Promoter for Ribulose-1, 5-bisphosphate Carboxylase/oxygenase Genes in Synechococcus sp. PCC7002 Found by an Improved Electrophoretic Mobility Shift Assay
• Statistical Analysis of the Relationship between Translation Initiation AUG Context and Gene Expression Level in Humans(Biochemistry & Molecular Biology)
• Microbial Decomposition of Organic Matter Derived from Phytoplankton Cellular Components in Seawater
• Phytoplankton Decomposition Process (PDP) Model Dealing with Carbon and Nitrogen Budget on Particulate Organic Matter
• Nutritional diagnosis of phytoplankton in Lake Baikal
• Decomposition of Phytoplankton in Seawater. Part 1: Kinetic Analysis of the Effect of Organic Matter Concentration
• Cryopreservation of Horseradish Hairy Root Cultures by Encapsulation-Dehydration
• Molecular Cloning and Partial Characterization of a Peroxidase Gene Expressed in the Roots of Portulaca oleracea cv., One Potentially Useful in the Remediation of Phenolic Pollutants
• Growth Inhibition of Various Organisms by a Violet Pigment, Nostocine A, Produced by Nostoc spongiaeforme
• Molecular Cloning and Partial Characterization of a Peroxidase Gene Expressed in the Roots of Portulaca oleracea cv., One Potentially Useful in the Remediation of Phenolic Pollutants
• Comparison of three Chlamydomonas strains which show distinctive oxidative stress tolerance(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Chemical Oxygen Demand Reduction using Lactic-acid Forming Bacteria to Treat River Mouth Sediment
• Characterization of Bisphenol A Metabolites Produced by Portulaca oleracea cv. by Liquid Chromatography Coupled with Tandem Mass Spectrometry

もっと見る 閉じる

スポンサーリンク