Cryopreservation of Horseradish Hairy Root Cultures by Encapsulation-Dehydration
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概要
- 論文の詳細を見る
Horseradish hairy root cultures were successfully cryopreserved by encapsulation-dehydration. Root tips excised from the cultures were encapsulated in Ca-alginate beads containing 0.5 M glycerol and 0.3 M sucrose, and then the beads were slowly dehydrated by air-drying over silica gel to reduce the water content to 33% prior to cooling in liquid nitrogen. Preculture and dehydration of the root tips in the presence of 2 μM abscisic acid was effective in increasing the survival rate, regeneration of hairy roots being observed in about 60% of the tips dehydrated and cooled in liquid nitrogen.
- 社団法人日本生物工学会の論文
- 1998-10-25
著者
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Du Dongning
Lab. Of Biotechnology Integrated Bioscience Section Graduate School Of Sci. And Technol. Shizuoka Un
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HIRATA KAZUMASA
Environmental Bioengineering Laboratory, Graduate School of Pharmaceutical Sciences, Osaka Universit
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MIYAMOTO KAZUHISA
Environmental Bioengineering Laboratory, Graduate School of Pharmaceutical Sciences, Osaka Universit
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Hirata Kazumasa
Environ. Bioeng. Lab Grad. Sch. Pharm. Sci. Osaka Univ.
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Miyamoto Kazuhisa
Environmental Bioengineering Laboratory Graduate School Of Pharmaceutical Sciences Osaka University
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GODA SAEKO
Environmental Bioengineering Laboratory, Graduate School of Pharmaceutical Sciences, Osaka Universit
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PHUNCHINDAWAN MANTHANA
Environmental Bioengineering Laboratory, Graduate School of Pharmaceutical Sciences, Osaka Universit
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DU DAN
Environmental Bioengineering Laboratory, Graduate School of Pharmaceutical Sciences, Osaka Universit
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ISHIO MASUMI
Environmental Bioengineering Laboratory, Graduate School of Pharmaceutical Sciences, Osaka Universit
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Goda Saeko
Environmental Bioengineering Laboratory Graduate School Of Pharmaceutical Sciences Osaka University
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Ishio Masumi
Environmental Bioengineering Laboratory Graduate School Of Pharmaceutical Sciences Osaka University
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Phunchindawan Manthana
Environmental Bioengineering Laboratory Graduate School Of Pharmaceutical Sciences Osaka University
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Miyamoto Kazuhisa
Environmental Bioengineering Laboratory Faculty Of Pharmaceutical Sciences Osaka University
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Hirata Kazumasa
Environmental Bioengineering Laboratory, Graduate School of Pharmaceutical Sciences, Osaka University
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