Molecular Cloning and Partial Characterization of a Peroxidase Gene Expressed in the Roots of Portulaca oleracea cv., One Potentially Useful in the Remediation of Phenolic Pollutants
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概要
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Portulaca (Portulaca oleracea cv.) efficiently removes phenolic pollutants from hydroponic solution. In plant roots, peroxidase (PRX) is thought to be involved in the removal of phenolic pollutants by the cross-linking them to cell wall polysaccharides or proteins at the expense of reduction of hydrogen peroxide (H2O2). In this study, we found that portulaca roots secreted an acidic PRX isozyme that had relatively high H2O2 affinity. We isolated five PRX genes, and the recombinant PRX proteins produced in cultured tobacco cells were partially characterized. Among these genes, PoPRX2 probably encoded the acidic PRX isozyme. PoPRX2 had an extra N-terminal region which has not been reported for other PRX proteins. We found that PoPRX2 oxidized phenolic pollutants, including bisphenol A, octylphenol, nonylphenol, and 17β-estradiol. In addition, we found that the Cys261 residue of PoPRX2 played an important role in the determination of affinity for H2O2 and stability toward H2O2.
- 2011-05-23
著者
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仲山 英樹
奈良先端科学技術大学大学院バイオサイエンス研究科
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Matsuura Hideyuki
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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仲山 英樹
奈良先端科学技術大学院大学バイオサイエンス研究科
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Hirata Kazumasa
Applied Environmental Biology Graduate School Of Pharmaceutical Sciences Osaka University
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Matsui Takeshi
Graduate School of Biological Sciences, Nara Institute of Science and Technology
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Nakayama Hideki
Graduate School of Biological Sciences, Nara Institute of Science and Technology
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Okuhata Hiroshi
The Kansai Electric Power Co. Environmental Research Center
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Kato K
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Kato Ko
Graduate School Of Biological Science Nara Institute Of Science And Technology
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Imai Sofue
Department Of Applied Environmental Biology Graduate School Of Pharmaceutical Sciences Osaka Univers
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Bamba Takeshi
Dep. Of Biotechnology Graduate School Of Engineering Osaka Univ.
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Bamba Takeshi
Department Of Applied Environmental Biology Graduate School Of Pharmaceutical Sciences Osaka Univers
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Harada Kazuo
Applied Environmental Biology Graduate School Of Pharmaceutical Sciences Osaka University
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仲山 かずや
Graduate School Of Biological Sciences Nara Institute Of Science And Technology:(present Office)orga
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Kawamura Kazue
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Miyasaka Hitosihi
The Kansai Electric Power Co. Environmental Research Center
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Miyasaka Hitoshi
Kansai Electric Power Co. Inc.
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Tsunemoto Mei
Department Of Environmental Biotechnology Graduate School Of Pharmaceutical Sciences Osaka Universit
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Maruta Takanori
Dep. Of Advanced Bioscience Fac. Of Agriculture Kinki Univ.
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Tamoi Masahiro
Department Of Advanced Bioscience Faculty Of Agriculture Kinki University
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Takagi Masahiro
School Of Material Science Japan Advanced Institute Of Science And Technology
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Miyasaka Hitoshi
The Kansai Electric Power Co. Environmental Res. Center Keihanna-plaza 12f 1-7 Seikacho Sourakugun K
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Hirata Kazumasa
Graduate School Of Pharmaceutical Sciences Osaka University
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Tanaka Satoshi
Research Institute For Environmental Sciences And Public Health Of Iwate Prefecture
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NOMURA Yuki
Graduate School of Biological Sciences, Nara Institute of Science and Technology (NAIST)
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TAKANO Mai
Graduate School of Biological Sciences, Nara Institute of Science and Technology (NAIST)
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Kato Ko
Graduate School Of Biological Sciences Nara Inst. Of Sci. And Technol. (naist)
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Tanaka Satoshi
Kansai Electric Power Co. Inc. Environmental Res. Center
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Okuhata Hiroshi
Kansai Electric Power Co. Inc. Environmental Res. Center
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Matsuura Hideyuki
Applied Environmental Biology Graduate School Of Pharmaceutical Sciences Osaka Univ.
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Hirata Kazumasa
Applied Environmental Biology Graduate School Of Pharmaceutical Sciences Osaka Univ.
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Imai Sofue
Applied Environmental Biology Graduate School Of Pharmaceutical Sciences Osaka Univ.
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Maruta Takanori
Department Of Food And Nutrition Faculty Of Agriculture Kinki University
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Takagi Masahiro
Graduate School Of Biological Sciences Nara Institute Of Science And Technology (naist)
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Nakayama Hideki
Graduate School Of Biological Sciences Nara Institute Of Science And Technology (naist)
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Sugio Tadatoshi
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Nomura Yuki
Graduate School Of Biological Sciences Nara Inst. Of Sci. And Technol. (naist)
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Nomura Yuki
Graduate School Of Biological Sciences Nara Institute Of Science And Technology (naist)
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仲山 英樹
神戸大・自・研究環
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仲山 英樹
神戸大学自然科学系先端融合研究環
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Matsui Takeshi
Graduate School Of Biological Sciences Nara Inst. Of Sci. And Technol. (naist)
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Matsui Takeshi
Advanced Technology Research Laboratories Idemitsu Kosan Co. Ltd.
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Okuhata Hiroshi
Kansai Electric Co. Environmental Research Center
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Miyasaka Hitoshi
Kansai Electric Co. Environmental Research Center
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Harada Kazuo
Applied Environmental Biology, Graduate School of Pharmaceutical Sciences, Osaka University
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