Establishment of evaluation method to determine effects of veterinary medicinal products on manure fermentation using small-scale composting apparatus(ENVIRONMENTAL BIOTECHNOLOGY)
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概要
- 論文の詳細を見る
To evaluate on a laboratory scale the influence of veterinary medicinal products (VMPs) excreted into feces on manure fermentation, we have developed an evaluation method that uses a small-scale composting apparatus. Each run is of approximately 3 kg scale and the operation can be conducted in an environmentally controlled laboratory. The main evaluation parameter is calorific value generated by aerobic fermentation. At the sulfadimethoxine (SDM) trial, the volume of CO_2 generated during fermentation and the disappearance of the inhibitory effect of immature manure on sprouting (using Komatsuna (Brassica rapa var. perviridis)) were measured. In addition, DNA of 16S rRNA was extracted from a manure sample and subjected to denaturing gradient gel electrophoresis (DGGE). The results suggest that the presence of such VMPs in feces affected the microbial community in manure fermentation, and indicate that the evaluation method may be used as a standard method to evaluate the effect of VMPs on the microbial community. Using the method, we obtained data of the influence of five VMPs approved for stockbreeding in Japan on swine manure fermentation. Erythromycin (EM) affected the caloriflc value even at a relatively low concentration (105 mg/3 kg manure). In contrast, oxytetracycline hydrochloride (OTC), norfloxacin (NFLX), and tylosin tartrate (TS) had no effect at that concentration. These VMPs also affected the increase of fermentation temperature when added at high concentrations.
著者
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Hirata Kazumasa
Applied Environmental Biology Graduate School Of Pharmaceutical Sciences Osaka University
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永瀬 裕康
Environmental Biotechnology Laboratory Graduate School Of Pharmaceutical Sciences Osaka University
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Otawa Kenichi
Graduate School Of Agricultural Science Tohoku University
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Hashimoto K
Environmental Bioengineering Laboratory Graduate School Of Pharmaceutical Sciences Osaka University
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Hirata Kazumasa
Department Of Biochemical Engineering Faculty Of Pharmaceutical Sciences Osaka University
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Hirata Kazumasa
Environmental Bioengineering Laboratory Graduated School Of Pharmaceutical Sciences Osaka University
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Hirata Kazumasa
Graduate School Of Pharm. Sci. Osaka Univ.
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Eguchi Kaoru
Environmental Bioengineering Laboratory Graduate School Of Pharmaceutical Sciences Osaka University
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Hirata K
Environmental Biotechnology Laboratory Graduate School Of Pharmaceutical Sciences Osaka University
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Hirata Kazumasa
Osaka Municipal Technical Research Institute
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Nagai Hidetaka
National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries
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Nakai Yutaka
Graduate School Of Agri Cultural Science Tohoku University
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Ogata Tomoko
National Veterinary Assay Laboratory Ministry Of Agriculture Forestry And Fisheries
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Eguchi Kaoru
National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries
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Ohishi Ryu
Graduate School of Agricultural Science, Tohoku University
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Nagase Hiroyasu
Graduate School of Pharmaceutical Science, Osaka University
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Murata Nanae
National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries
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Ishikawa Hitoshi
Research Team for Bacterial/Parasitic Disease, National Institute of Animal Health
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Ohishi Ryu
Graduate School Of Agricultural Science Tohoku University
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Murata Nanae
National Veterinary Assay Laboratory Ministry Of Agriculture Forestry And Fisheries
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Eguchi Kaoru
National Veterinary Assay Laboratory Ministry Of Agriculture Forestry And Fisheries
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Nagai Hidetaka
National Veterinary Assay Laboratory Ministry Of Agriculture Forestry And Fisheries
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