Isolation of polyphenol oxidase genes from Portulaca oleracea and evaluation of their ability to metabolize endocrine-disrupting chemicals
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概要
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Portulaca (Portulaca oleracea cv.), a garden plant, efficiently removes endocrine-disrupting chemicals (EDCs) including bisphenol A (BPA) from hydroponic solution. We hypothesized that polyphenol oxidase (PPO) was involved in the initial steps of detoxifying EDCs in portulaca roots. In order to elucidate the molecular basis of portulaca’s ability to metabolize EDCs, we first isolated five PPO genes (PoPPO1–5) that were expressed mainly in portulaca roots. Among these genes, PoPPO2, PoPPO4 and PoPPO5 were introduced into cultured tobacco cells and expressed as active forms. We found that crude extracts from the cells expressing PoPPO2, PoPPO5, and to a lesser extent PoPPO4, could metabolize BPA. In addition, we found that the BPA metabolites from crude extracts of cells expressing PoPPO2, PoPPO4 and PoPPO5 were identical to those of portulaca. Moreover, PoPPO2 and PoPPO5 also caused hydroxylation of octylphenol, nonylphenol and 17β-estradiol. Therefore, these results strongly suggest that PoPPOs significantly contribute to the superior ability of portulaca to metabolize EDCs.
著者
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Kato Ko
Graduate School Of Biological Science Nara Institute Of Science And Technology
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Harada Kazuo
Graduate School Of Pharmaceutical Sciences Osaka Univ.
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Hirata Kazumasa
Graduate School Of Pharm. Sci. Osaka Univ.
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TANAKA Satoshi
Environmental Research Center, Kansai Electric Power Co.
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MIYASAKA Hitoshi
Environmental Research Center, Kansai Electric Power Co.
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Matsuura Hideyuki
Graduate School Of Agriculture Hokkaido University
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Hirata Kazumasa
Graduate School Of Pharmaceutical Sciences Osaka University
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Maruta Takanori
Department Of Food And Nutrition Faculty Of Agriculture Kinki University
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Nakayama Hideki
Graduate School Of Biological Sciences Nara Institute Of Science And Technology (naist)
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Matsui Takeshi
Graduate School Of Biological Sciences Nara Inst. Of Sci. And Technol. (naist)
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Kaneda Hirokazu
Graduate School of Pharmaceutical Sciences, Osaka University
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Tomiyasu Ryosuke
Graduate School of Pharmaceutical Sciences, Osaka University
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Kuroda Yukako
Graduate School of Pharmaceutical Sciences, Osaka University
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Higashimoto Yuka
Graduate School of Pharmaceutical Sciences, Osaka University
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Oda Tomoka
Graduate School of Pharmaceutical Sciences, Osaka University
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Okuhata Hiroshi
Environmental Research Center, The Kansai Electric Power Co., Inc.
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Tanaka Satoshi
Environmental Research Center, The Kansai Electric Power Co., Inc.
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NAKAYAMA Hideki
Graduate School of Fisheries Science and Environmental Studies, Nagasaki University
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MATSUURA Hideyuki
Graduate School of Pharmaceutical Sciences, Osaka University
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