Allene oxide cyclase is essential for theobroxide-induced jasmonic acid biosynthesis in Pharbitis nil
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概要
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Theobroxide, a natural product, strongly stimulates the biosynthesis of jasmonic acid (JA) in Pharbitis nil. In this study, we investigated the accumulation of protein by the immunoblot analysis of lipoxygenase (LOX), allene oxide synthase (AOS) and allene oxide cyclase (AOC), key enzymes in JA biosynthesis, and how the endogenous levels of JA in Pharbitis nil are affected by theobroxide. The effect of JA on the accumulations of these proteins was monitored simultaneously. The results show that theobroxide treatment led to a high level accumulation of JA, which is due to high accumulations of LOX, AOS and AOC proteins induced by theobroxide treatment both under short day (SD) and long day (LD) conditions. However, under SD conditions AOS and AOC proteins are not enhanced by JA treatment. Kinetic analysis of protein levels show that a biphasic activation of AOC protein by theobroxide is displayed and the first activation of AOC protein together with elevated JA levels are observed within 30 minutes after treatment. Meanwhile, AOS and LOX proteins are activated by theobroxide later than AOC protein, suggesting that AOC plays an essential role in the initial JA formation induced by theobroxide. Since theobroxide-increased JA levels also show a biphasic manner similar to AOC activation and AOS, LOX proteins are activated later than AOC, and thus we propose a positive JA feedback regulation.Interestingly, AOS protein, which is also the enzyme for the biosynthesis of 9,10-ketol-octadecadienoic acid (KODA, a flowering inducing factor), accumulates markedly due to the simultaneous involvement of theobroxide and SD conditions, suggesting that AOS probably plays a role in flower bud formation in Pharbitis nil.
- Elsevierの論文
- 2005-11-04
著者
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Nabeta Kensuke
Hokkaido Univ. Sapporo Jpn
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Matsui Hirokazu
Dept. Biosci. Chem. Fac. Agric. Hokkaido Univ.
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Matsui Hirokazu
Hokkaido Univ. Sapporo Jpn
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Matsuura Hideyuki
Laboratory Of Bioorganic Chemistry Division Of Applied Life Science Graduate School Of Agriculture H
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Yoshihara Teruhiko
Lab. Of Bioorganic Chemistry Div. Of Applied Bioscience Graduate School Of Agriculture Hokkaido Univ
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KONG Fan-Jiang
Research Faculty of Agriculture, Hokkaido University
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YOSHIHARA Teruhiko
Research Faculty of Agriculture, Hokkaido University
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Matsuura Hideyuki
Graduate School Of Agriculture Hokkaido University
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Matsuura Hideyuki
Hokkaido Univ. Sapporo
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Kong Fan-jiang
Research Faculty Of Agriculture Hokkaido University
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Takahashi Kosaku
Div. Of Applied Bioscience Res. Fac. Of Agriculture Hokkaido Univ. Kita-9 Nishi-9 Kita-ku Sapporo 06
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Abe Jun
Research Faculty Of Agriculture Hokkaido University
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Yoshihara Teruhiko
Research Faculty Of Agriculture Hokkaido University
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