Chemical Modification of β-Glucosidase/β-Fucosidase from Dalbergia cochinchinensis Pierre by Conduritol B Epoxide

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概要

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• Studies have been done on the inhibition and inactivation of the β-glucosidase and β=fucosidase enzyme from Thai Rosewood (Dalbergia cochinchinesis Pierre). The enzyme was inhibited by Tris with similar K_i of 11.7 mM and 14.3 mM for the hydrolysis of p-nitrophenyl β-D-glucoside (PNPG) and p-nitrophenyl β-D-fucoside (PNPF), respectively. Conduritol B epoxide inhibited both β-glucosidase and β-fucosidase activities to similar extents, with a pseudo-first-order rate constant (k_<obs>) of inactivation of 5.56×10<-3>s<-1>, and binding stoichiometry of 0.9 mol per subunit. Partially inactivated enzyme showed similar kinetics with PNPG and PNPF as substrates. Moreover, Tris at 300mM protected both β-glucosidase and β-fucosidase activities from inactivation by 6mM CBE. The data support the idea that the Dalbergia cochinchinensis Pierre enzyme has a common active site for the hydrolysis of PNPG and PNPF.

• 社団法人日本農芸化学会の論文
• 1996-08-23

著者

• Matsui Hirokazu

  Department of Bioscience and Chemistry, Faculty of Agriculture, Hokkaido University

• SURARIT Rudee

  Faculty of Dentistry, Mahidol University

• Chiba Seiya

  Department Of Agricultural Chemistry Faculty Of Agriculture Hokkaido University

• Chiba Seiya

  Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University

• Matsui Hirokazu

  Dept. Biosci. Chem. Fac. Agric. Hokkaido Univ.

• Matsui Hirokazu

  Department Of Applied Bioscience Graduate School Of Agriculture Hokkaido University

• SRISOMSAP Chantragan

  Laboratory of Biochemistry, Chulabhorn Research Institute

• Svasti Jisnuson

  Department Of Biochemistry Faculty Of Science Center For Excellence In Protein Structure And Functio

• Svasti Jisnuson

  Department Of Biochemistry And Center For Excellence In Protein Structure & Function Faculty Of

• Srisomsap Chantragan

  Laboratory Of Biochemistry Chulabhorn Research Institute

• Surarit Rudee

  Faculty Of Dentistry Mahidol University

• Surarit Rudee

  Department of Physiology and Biochemistry, Faculty of Dentistry, Mahidol University

• Svasti J

  Faculty Of Science Mahidol University

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