Evidence for a Single Active Site in β-D-Glucosidase/β-D-Fucosidase from Dalbergia cochinchinensis Seeds
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概要
- 論文の詳細を見る
Kinetic analyses have been done on the hydrolysis of p-nitrophenyl β-D-glucoside (PNPG) and P-nitrophenyl β-D-fucoside (PNPF) by the β-D-glucosidase/β-D-fucosidase enzyme from Thai Rosewood (Dalbergia cochinchinensis Pierre). PNPF showed a competitive inhibition of PNPG hydrolysis with a K_i of 0.42 mM. Hydrolysis of mixtures of PNPG and PNPF at fractional ratios ranging from 0 to 1 showed Lineweaver-Burk plots intermediate between the two extremes. The apparent K_m and apparent V_<max> at each fractional ratio showed good correspondence with the theoretical curves predicted for the existence of a single common active site for the hydrolysis of the two substrates.
- 社団法人日本農芸化学会の論文
- 1997-01-23
著者
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Matsui Hirokazu
Department of Bioscience and Chemistry, Faculty of Agriculture, Hokkaido University
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千葉 誠哉
Graduate School Of Agriculture Hokkaido University
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SURARIT Rudee
Faculty of Dentistry, Mahidol University
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Chiba Seiya
Department Of Agricultural Chemistry Faculty Of Agriculture Hokkaido University
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Chiba Seiya
Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
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Matsui Hirokazu
Dept. Biosci. Chem. Fac. Agric. Hokkaido Univ.
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Matsui Hirokazu
Department Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
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SRISOMSAP Chantragan
Laboratory of Biochemistry, Chulabhorn Research Institute
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Svasti Jisnuson
Department Of Biochemistry Faculty Of Science Center For Excellence In Protein Structure And Functio
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Svasti Jisnuson
Department Of Biochemistry And Center For Excellence In Protein Structure & Function Faculty Of
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Srisomsap Chantragan
Laboratory Of Biochemistry Chulabhorn Research Institute
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Surarit Rudee
Faculty Of Dentistry Mahidol University
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Surarit Rudee
Department of Physiology and Biochemistry, Faculty of Dentistry, Mahidol University
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Svasti J
Faculty Of Science Mahidol University
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MATSUI Hirokazu
Department of Agricultural Chemistry, Faculty of Agriculture, Hokkaido University
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