Cloning and Sequencing of a cDNA Encoding α-Glucosidase from Sugar Beet
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概要
- 論文の詳細を見る
A cDNA encoding sugar beet α-glucosidase was cloned from a library constructed from mRNA of suspension-cultured cells. The cDNA, 3056bp in length, had an open reading frame encoding a polypeptide of 913 amino acid residues with a molecular mass of 102,078 Da, included only one of four regions which were conserved in the α-amylase family of enzymes. The deduced amino acid sequence from the analysis of the cDNA contained the sequences of the proteolysis peptides and the active site region peptide of sugar beet α-glucosidase. The primary structure indicated relatively high homology in the range of 28.2 to 54.3% to those for other α-glucosidases. The highest homology was found in barley α-glucosidase.
- 社団法人日本農芸化学会の論文
- 1997-05-23
著者
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森 春英
北海道大学大学院農学研究科応用生命科学専攻
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Ito Hiroyuki
Faculty of Agriculture, Hokkaido University
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Matsui Hirokazu
Faculty of Agriculture, Hokkaido University
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千葉 誠哉
Graduate School Of Agriculture Hokkaido University
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Chiba Seiya
Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
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HONMA Mamoru
Faculty of Agriculture, Hokkaido University
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Ito Hiroyuki
Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
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IWANAMI Shunsuke
Faculty of Agriculture, Hokkaido University
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MORI Haruhide
Faculty of Agriculture, Hokkaido University
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CHIBA Seiya
Faculty of Agriculture, Hokkaido University
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Matsui Hirokazu
Dept. Biosci. Chem. Fac. Agric. Hokkaido Univ.
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Honma M
Department Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
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Honma Mamoru
Faculty Of Agriculture Hokkaido University
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Ito H
Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
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Inoue S
Hokkaido Univ. Sapporo Jpn
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