Chemical Modification and Amino Acid Sequence of Active Site in Sugar Beet α-Glucosidase

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概要

• 論文の詳細を見る

• The modification of amino acid residues in sugar beet α-glucosidase with conduritol B epoxide (CBE), an affinity labeling reagent, inactivated the enzyme. The inactivation followed pseudo-first-order kinetics. The enzyme was protected from inactivation by a competitive inhibitor, Tris, and the partially inactivated enzymes showed only the decrease of V values and no change in Km value. An ^3H-CBE labeled peptide isolated from the digest of the inactivated enzyme with Lys-C protease was sequenced. The -COO - group of Asp was found to be specifically labeled, implicating that it is a catalytic group of the enzyme. The sequence around the essential Asp was determined to be -DGIWIDMNE-, which showed a high homology with those of other α-glucosidases.

• 社団法人日本農芸化学会の論文
• 1995-03-23

著者

• 森 春英

  北海道大学大学院農学研究科応用生命科学専攻

• Ito Hiroyuki

  Faculty of Agriculture, Hokkaido University

• Matsui Hirokazu

  Faculty of Agriculture, Hokkaido University

• Ito H

  National Agricultural Res. Organization Mie Jpn

• 木村 淳夫

  北海道大学大学院農学研究院応用生命科学部門

• Chiba Seiya

  Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University

• HONMA Mamoru

  Faculty of Agriculture, Hokkaido University

• Ito Hiroyuki

  Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University

• IWANAMI Shunsuke

  Faculty of Agriculture, Hokkaido University

• MORI Haruhide

  Faculty of Agriculture, Hokkaido University

• CHIBA Seiya

  Faculty of Agriculture, Hokkaido University

• Kimura Atsuo

  Faculty of Agriculture, Hokkaido University

• Matsui Hirokazu

  Dept. Biosci. Chem. Fac. Agric. Hokkaido Univ.

• Honma M

  Department Of Applied Bioscience Graduate School Of Agriculture Hokkaido University

• Honma Mamoru

  Faculty Of Agriculture Hokkaido University

• Ito H

  Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University

• Inoue S

  Hokkaido Univ. Sapporo Jpn

• Kimura Atsuo

  Hokkaido University

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