α-Glucosidase Mutant Catalyzes "α-Glycosynthase"-type Reaction(Biochemistry & Molecular Biology)

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概要

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• Replacement of the catalytic nucleophile Asp481 by glycine in Schizosaccharomyces pombe α-glucosidase eliminated the hydrolytic activity. The mutant enzyme (D481G) was found to catalyze the formation of an α-glucosidic linkage from β-glucosyl fluoride and 4-nitrophenyl (PNP) α-glucoside to produce two kinds of PNP α-diglucosides, α-isomaltoside and α-maltoside. The two products were not hydrolyzed by D481G, giving 41 and 29% yields of PNP α-isomaltoside and α-maltoside, respectively. PNP monoglycosides, such as α-xyloside, α-mannoside, or β-glucoside, acted as the substrate, but PNP α-galactoside and maltose could not. No detectable product was observed in the combination of α-glucosyl fluoride and PNP α-glucoside. This study is the first report on an "α-glycosynthase"-type reaction to form an α-glycosidic linkage.

• 社団法人日本農芸化学会の論文
• 2002-04-23

著者

• 森 春英

  北海道大学大学院農学研究科応用生命科学専攻

• 森 春英

  北海道大学大学院農学研究院応用生命科学部門

• 千葉 誠哉

  Graduate School Of Agriculture Hokkaido University

• 木村 淳夫

  北海道大学大学院農学研究院応用生命科学部門

• MORI Haruhide

  Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University

• OKUYAMA Masayuki

  Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University

• KIMURA Atsuo

  Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University

• CHIBA Seiya

  Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University

• WATANABE Kotomi

  Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University

• Chiba Seiya

  Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University

• Matsui Hirokazu

  Dept. Biosci. Chem. Fac. Agric. Hokkaido Univ.

• Matsui Hirokazu

  Hokkaido Univ. Sapporo Jpn

• Kobayasi Kazuhiro

  Department Of Agriculture Faculty Of Life And Environmental Science Shimane University

• Kimura Atsuo

  Hokkaido Univ.

• Kimura Atsuo

  Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University

• Kimura Atsuo

  Graduate School Of Agriculture Hokkaido University

• Watanabe Kotomi

  Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University

• Mori Haruhide

  Division Of Applied Bioscience Research Faculty Of Agriculture Hokkaido University

• Okuyama Masayuki

  Research Faculty Of Agriculture Hokkaido University

• Okuyama Masayuki

  Division Of Applied Bioscience Research Faculty Of Agriculture Hokkaido University

• Kimura Atsuo

  Hokkaido University

• Matsui Hirokazu

  Laboratory Of Biochemistry Research Faculty Of Agriculture Hokkaido University

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