Molecular Cloning of cDNA for Trehalase from the European Honeybee, Apis mellifera L., and Its Heterologous Expression in Pichia pastoris
スポンサーリンク
概要
- 論文の詳細を見る
cDNA encoding the bound type trehalase of the European honeybee was cloned. The cDNA (3,001 bp) contained the long 5′ untranslated region (UTR) of 869 bp, and the 3′ UTR of 251 bp including a poly(A) tail, and the open reading frame of 1,881 bp consisting of 626 amino acid residues. The Mr of the mature enzyme comprised of 591 amino acids, excluded a signal sequence of 35 amino acid residues, was 69,177. Six peptide sequences analyzed were all found in the deduced amino acid sequence. The amino acid sequence exhibited high identity with trehalases belonging to glycoside hydrolase family 37. A putative transmembrane region similar to trehalase-2 of the silkworm was found in the C-terminal amino acid sequence. Recombinant enzyme of the trehalase was expressed in the methylotrophic yeast Pichia pastoris as host, and displayed properties identical to those of the native enzyme except for higher sugar chain contents. This is the first report of heterologous expression of insect trehalase.
- 社団法人 日本農芸化学会の論文
著者
-
森 春英
北海道大学大学院農学研究院応用生命科学部門
-
KIM Doman
School of Biological Sciences and Technology and Institute of Bioindustrial Technology, Jeonnam Nati
-
NISHIMOTO Mamoru
Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University
-
MORI Haruhide
Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University
-
OKUYAMA Masayuki
Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University
-
WONGCHAWALIT Jintanart
Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University
-
KIMURA Atsuo
Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University
-
CHIBA Seiya
Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University
-
LEE Jin-Ha
Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University
-
Chiba Seiya
Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
-
Saito Saori
Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University
-
Matsui Hirokazu
Dept. Biosci. Chem. Fac. Agric. Hokkaido Univ.
-
Matsui Hirokazu
Hokkaido Univ. Sapporo Jpn
-
Kim Doman
School Of Biological Sciences And Technology And Institute Of Bioindustrial Technology Jeonnam Natio
-
Kimura Atsuo
Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
-
Mori Haruhide
Division Of Applied Bioscience Research Faculty Of Agriculture Hokkaido University
-
Saito Saori
Division Of Applied Bioscience Research Faculty Of Agriculture Hokkaido University
-
Okuyama Masayuki
Division Of Applied Bioscience Research Faculty Of Agriculture Hokkaido University
-
Nishimoto Mamoru
Division Of Applied Bioscience Research Faculty Of Agriculture Hokkaido University
-
Lee Jin-ha
Research Faculty Of Agriculture Hokkaido University
-
Matsui Hirokazu
Laboratory Of Biochemistry Research Faculty Of Agriculture Hokkaido University
-
Wongchawalit Jintanart
Division Of Applied Bioscience Research Faculty Of Agriculture Hokkaido University
-
Lee Jin-ha
Division Of Applied Bioscience Research Faculty Of Agriculture Hokkaido University
-
LEE Jin-Ha
Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University
-
Kim Doman
School of Biological Science and Biotechnology, Chonnam National University
関連論文
- Identification of a β-glucosidase hydrolyzing tuberonic acid glucoside in rice (Oryza sativa L.)
- イソマルトデキストラナーゼのサブサイト親和力の評価
- Brevibacterium fuscum var. dextranlyticum 0407 株由来α-グルコシダーゼ様遺伝子の単離と配列分析
- α-1,6およびα-1,4グルコシド結合に加水分解活性を有するイソマルトデキストラナーゼの活性部位の検討
- トラマメ(Phaseolus vulgaris L.)発芽子葉α-アミラーゼcDNAのクローニング
- トラマメ登熟種子中におけるアミラーゼおよび枝つけ酵素
- テンサイα-グルコシダーゼの基質特異性と全一次構造
- Aspergillus niger結晶α-グルコシダーゼの一次構造と糖鎖
- Brevibacterium fuscum var. dextranlyticum由来イソマルトトリオデキストラナーゼの諸性質の再検討
- Aspergillus oryzae由来の3種のα-グルコシダーゼの性質
- コメα-グルコシダーゼIIの活性解離基の同定
- テンサイα-Glucosidaseの一次構造について : 酵素
- トラマメ(Phaseolus vulgaris L.)の発芽子葉及び緑葉で発現するα-amylaseについて : 酵素
- トラマメ(Phaseolus vulgaris L. cv Toramame)発芽子葉α-Amylaseの基質特異性とサブサイト親和力
- イソマルトデキストラナーゼによる酸処理デキストランからのイソマルトースの新しい調製法
- 植物α-グルコシダーゼ, 特にイネ酵素の分子解析と発芽時の澱粉粒分解に関する研究
- Cloning and sequencing of the cellobiose 2-epimerase gene from an obligatory anaerobe, Ruminococcus albus.
- 植物α-アミラーゼの機能と構造に関する研究
- Molecular Cloning of cDNAs and Genes for Three α-Glucosidases from European Honeybees, Apis mellifera L., and Heterologous Production of Recombinant Enzymes in Pichia pastoris
- Site-directed mutagenesis of possible catalytic residues of cellobiose 2-epimerase from Ruminococcus albus
- Streptococcus mutans Dextran Glucosidase の基質認識機構
- Isomaltotrio-dextranase Iの活性解離基について : 酵素
- Synthesis of Thermo- and Acid-stable Novel Oligosaccharides by Using Dextransucrase with High Concentration of Sucrose
- 大腸菌 YicI(α-xylosidase) の基質認識機構
- Function-unknown Glycoside Hydrolase Family 31 Proteins, mRNAs of which were Expressed in Rice Ripening and Germinating Stages, are α-Glucosidase and α-Xylosidase
- Crystallization and preliminary X-ray analysis of Streptococcus mutans dextran glucosidase
- Multiple forms of α-glucosidase in rice seeds (Oryza sativa L., var Nipponbare)
- Purification and Characterization of α-Glucosidase I from Japanese Honeybee (Apis cerana japonica) and Molecular Cloning of Its cDNA
- Glucoamylase Originating from Schwanniomyces occidentalis Is a Typical α-Glucosidase
- Localization of α-Glucosidases I, II, and III in Organs of European Honeybees, Apis mellifera L., and the Origin of α-Glucosidase in Honey
- α-Glucosidase 変異酸素が α-Glycosynthase 反応を触媒する
- Purification, Characterization, and Sequence Analysis of Two α-Amylase Isoforms from Azuki Bean, Vigna angularis, Showing Different Affinity towards β-Cyclodextrin Sepharose(Biochemistry & Molecular Biology)
- α-グルコシダーゼによって触媒される分子内糖転移反応の証明
- Identification of Essential lonizable Groups and Evaluation of Subsite Affinities in the Active Site of β-D-Glucosidase F_1 from a Streptomyces sp.(Biochemistry & Molecular Biology)
- Streptomyces sp. β-_D-グルコシダーゼF_1の基質特異性および活性部位に関する反応速度論的解析
- α-Glucosidase Mutant Catalyzes "α-Glycosynthase"-type Reaction(Biochemistry & Molecular Biology)
- Family IIに属するα-Glucosidaseのプロトン供与体としての触媒アミノ酸残基
- 3種のミツバチα-グルコシダーゼアイソザイムの構造と機能
- Purification and Identification of the Essential lonizable Groups of Honeybee, Apis mellifera L., Trehalase(Biochemistry & Molecular Biology)
- 酵母α-グルコシダーゼの存在部位の解析
- Cloning and Heterologous Expression of a β-Fructofuranosidase Gene from Arthrobacter globiformis IFO 3062, and Site-Directed Mutagenesis of the Essential Aspartic Acid and Glutamic Acid of the Active Site (ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHN
- Cloning and Heterologous Expression of a Glucodextranase Gene from Arthrobacter globiformis I42, and Experimental Evidence for the Catalytic Diad of the Recombinant Enzyme(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Cloning of a Gene Cluster Responsible for the Biosynthesis of Diterpene Aphidicolin, a Specific Inhibitor of DNA Polymerase α
- Gibberellin Is Essentially Required for Carrot (Daucus carota L.) Somatic Embryogenesis : Dynamic Regulation of Gibberellin 3-Oxidase Gene Expressions(Biochemistry & Molecular Biology)
- A Wound-Inducible Tobacco Peroxidase Gene Expresses Preferentially in the Vascular System
- Purification and Substrate Specificity of Honeybee, Apis mellifera L., α-Glucosidase III(Biochemistry & Molecular Biology)
- 1-Aminocyclopropane-1-carboxylate Synthase of Penicillium citrinum : Primary Structure and Expression in Escherichia coli and Saccharomyces cerevisiae(Biochemistry & Molecular Biology)
- Molecular Cloning of Isomaltotrio-dextranase Gene from Brevibacterium fuscum var. dextranlyticum strain 0407 and Its Expression in Escherichia coli
- A Catalytic Amino Acid and Primary Structure of Active Site in Aspergillus niger α-Glucosidase
- ミツバチTrehalaseによる水解生成物のアノマー型の分析(酵素-糖質関連酵素-)
- ミツバチ結合型Treharaseの精製と諸性質について(酵素-糖質関連酵素-)
- ω-エポキシアルキルα-グルコシドによるグリコシダーゼの親和標識
- Cloning and Sequencing of a cDNA Encoding α-Glucosidase from Sugar Beet
- Molecular Cloning and Nucleotide Sequences of cDNA and Gene Encoding endo-Inulinase from Penicillium purpurogenum
- Chemical Modification and Amino Acid Sequence of Active Site in Sugar Beet α-Glucosidase
- Complete Amino Acid Sequence of Crystalline α-Glucosidase from Aspergillus niger
- Substrate Specificity and Subsite Affinities of Crystalline α-Glucosidase from Aspergillus niger(Biological Chemistry)
- Aspergillus niger GRM 3 α-グルコシダーゼの諸性質と基質特異性
- Catalytic Reaction Mechanism Based on α-Secondary Deuterium Isotope Effects in Hydrolysis of Trehalose by European Honeybee Trehalase
- Molecular Cloning of cDNA for Trehalase from the European Honeybee, Apis mellifera L., and Its Heterologous Expression in Pichia pastoris
- オオムギα-アミラーゼの基質認識のメカニズムを探る
- Barley Proteome Analysis, Starch Degrading Eznymes and Proteinaceous Inhibitors
- Impact on Substrate Specificity of Mutational Subsite Isozyme Mimicry in Barley α-Amylase
- α-GlucosidaseのD-Glucal水和反応の立体化学(酵素-糖質関連酵素-)
- Interactions between Barley α-Amylases, Substrates, Inhibitors and Regulatory Proteins
- アイソト-プ効果によるエキソ-α-グルカナ-ゼの反応機構の解析 (アミラ-ゼシンポジウム(1990)特集号)
- 4.Mucor属糸状菌のCysteine-conjugateβ-lyase(ビタミンB_6酵素を楽しむ会研究発表要旨,ビタミンB_6酵素研究会)
- Molecular Cloning, Functional Expression, and Tissue Distribution of a Potato Sprout Allene Oxide Synthase Involved in a 9-Lipoxygenase Pathway
- 植物α-Glucosidaseの活性中心 : 特にテンサイについて : 酵素
- The N-terminal region of the starch-branching enzyme from Phaseolus vulgaris L. is essential for optimal catalysis and structural stability
- ω-エポキシアルキルα-D-グルコピラノシドによるエンド型デキストラナーゼの自殺基質型失活
- Characterization of Starch Synthase I and II Expressed in Early Developing Seeds of Kidney Bean (Phaseolus vulgaris L.)
- Purification and Partial Characterization of a Novel Glucanhydrolase from Lipomyces starkeyi KSM 22 and its Use for Inhibition of Insoluble Glucan Formation
- OsJAR1 and OsJAR2 are jasmonyl-L-isoleucine synthases involved in wound- and pathogen-induced jasmonic acid signalling
- α-グルコシダーゼの分子解析
- Practical Preparation of Epilactose Produced with Cellobiose 2-Epimerase from Ruminococcus albus NE1
- Molecular Cloning of cDNAs and Genes for Three α-Glucosidases from European Honeybees, Apis mellifera L., and Heterologous Production of Recombinant Enzymes in Pichia pastoris
- Catalytic Reaction Mechanism Based on α-Secondary Deuterium Isotope Effects in Hydrolysis of Trehalose by European Honeybee Trehalase
- Identification of the Cellobiose 2-Epimerase Gene in the Genome of Bacteroides fragilis NCTC 9343
- ビールの国でのオオムギα-アミラーゼ研究 デンマーク・カールスバーグ研究所に滞在して
- Isolation and Structural Elucidation of a New Cyclohexenone Compound from Lasiodiplodia theobromae
- Potato Tuber Cell Expansion-Inducing Activity of Stereochemically Restricted JA Analogs
- Cloning and Functional Analysis of an Allene Oxide Synthase in Physcomitrella patens
- Kinetics of the Accumulation of Jasmonic Acid and Its Derivatives in Systemic Leaves of Tobacco (Nicotiana tabacum cv. Xanthi nc) and Translocation of Deuterium-Labeled Jasmonic Acid from the Wounding Site to the Systemic Site
- Comparison of Enzymatic Properties and Gene Expression Profiles of Two Tuberonic Acid Glucoside β-Glucosidases from Oryza sativa L.
- Calcium Ion-Dependent Increase in Thermostability of Dextran Glucosidase from Streptococcus mutans
- Cellulase Applied to the Leaves of Sweet Pepper (Capsicum annuum L. var. grossum) Upregulates the Production of Salicylic and Azelaic Acids
- Biosynthesis of Resorcylic Acid Lactone (5S)-5-Hydroxylasiodiplodin in Lasiodiplodia theobromae
- Biosynthesis of Resorcylic Acid Lactone Lasiodiplodin in Lasiodiplodia theobromae
- Crystallization and preliminary crystallographic analysis of dextranase from Streptococcus mutans
- Calcium ion-dependent increase in thermostability of dextran glucosidase from Streptococcus mutans.
- Truncation of N- and C-terminal regions of Streptococcus mutans dextranase enhances catalytic activity.
- Physiological and Biochemical Characterization of Three Nucleoside Diphosphate Kinase Isozymes from Rice (Oryza sativa L.)
- Distal Transport of Exogenously Applied Jasmonoyl-Isoleucine with Wounding Stress
- Tobacco Salicylic Acid Glucosyltransferase Is Active toward Tuberonic Acid (12-Hydroxyjasmonic Acid) and Is Induced by Mechanical Wounding Stress
- Arabidopsis CYP94B3 Encodes Jasmonyl-L-Isoleucine 12-Hydroxylase, a Key Enzyme in the Oxidative Catabolism of Jasmonate
- The Delay in the Development of Experimental Colitis from Isomaltosyloligosaccharides in Rats Is Dependent on the Degree of Polymerization
- Metabolic Mechanism of Mannan in a Ruminal Bacterium, Ruminococcus albus, Involving Two Mannoside Phosphorylases and Cellobiose 2-Epimerase : Discovery of a New Carbohydrate Phosphorylase, β-1,4-Mannooligosaccharide Phosphorylase
- セロビオース2-エピメラーゼを用いたエピラクトースの実用的酵素合成法の開発(応用糖質科学シンポジウム)
- オリゴ糖の製法開発と食品への応用, 監修:早川幸男,中久喜輝夫, シーエムシー出版