Multiple forms of α-glucosidase in rice seeds (Oryza sativa L., var Nipponbare)

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概要

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• Two isoforms of α-glucosidases (ONG2-I and ONG2-II) were purified from dry rice seeds (Oryza sativa L., var Nipponbare). Both ONG2-I and ONG2-II were the gene products of ONG2 mRNA expressed in ripening seeds. Each enzyme consisted of two components of 6 kDa-peptide and 88 kDa-peptide encoded by this order in ONG2 cDNA (ong2), and generated by post-translational proteolysis. The 88 kDa-peptide of ONG2-II had 10 additional N-terminal amino acids compared with the 88 kDa-peptide of ONG2-I. The peptides between 6 kDa and 88 kDa components (26 amino acids for ONG2-I and 16 for ONG2-II) were removed by post-translational proteolysis. Proteolysis induced changes in adsorption and degradation of insoluble starch granules. We also obtained three α-glucosidase cDNAs (ong1, ong3, and ong4) from ripening seeds. The ONG1, ONG2, and ONG4 genes were situated in distinct locus of rice genome. The transcripts encoding ONG2 and ONG3 were generated by alternative splicing. Members of α-glucosidase multigene family are differentially expressed during ripening and germinating stages in rice.

• Elsevier Masson SASの論文

著者

• 森 春英

  北海道大学大学院農学研究科応用生命科学専攻

• 森 春英

  北海道大学大学院農学研究院応用生命科学部門

• Sano Yoshio

  Graduate School of Agriculture, Hokkaido University

• Kim Young-min

  Graduate School Of Agriculture Hokkaido University

• Nakai Hiroyuki

  Graduate School Of Agriculture Hokkaido University

• 千葉 誠哉

  Graduate School Of Agriculture Hokkaido University

• 木村 淳夫

  北海道大学大学院農学研究院応用生命科学部門

• ITO Tatsuya

  Graduate School of Agriculture, Hokkaido University

• KAMIYA Koutarou

  Graduate School of Agriculture, Hokkaido University

• MATSUBARA Kazuki

  Graduate School of Agriculture, Hokkaido University

• WONGCHAWALIT Jintanart

  Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University

• Kamiya Koutarou

  Graduate School Of Agriculture Hokkaido University

• Chiba Seiya

  Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University

• Matsui Hirokazu

  Dept. Biosci. Chem. Fac. Agric. Hokkaido Univ.

• Matsui Hirokazu

  Hokkaido Univ. Sapporo Jpn

• Matsubara Kazuki

  Graduate School Of Agriculture Hokkaido University

• Kobayasi Kazuhiro

  Department Of Agriculture Faculty Of Life And Environmental Science Shimane University

• Sano Yoshio

  Graduate School Of Agriculture Hokkaido University

• Kimura Atsuo

  Hokkaido Univ.

• Kimura Atsuo

  Graduate School Of Agriculture Hokkaido University

• Kim Young-min

  Hokkaido Univ.

• Kim Young-min

  Hokkaido University

• Kim Young-min

  Special Business Division Koreaabrasive Ind. Co. Ltd.

• Okuyama Masayuki

  Research Faculty Of Agriculture Hokkaido University

• Kimura Atsuo

  Hokkaido University

• Matsui Hirokazu

  Laboratory Of Biochemistry Research Faculty Of Agriculture Hokkaido University

• Ito Tatsuya

  Graduate School Of Agriculture Hokkaido University

• Yamamoto Takeshi

  Graduate School Of Agriculture Hokkaido University

• Mori Haruhide

  Research Faculty Of Agriculture Hokkaido University

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