Crystallization and preliminary X-ray analysis of Streptococcus mutans dextran glucosidase

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概要

• 論文の詳細を見る

• Dextran glucosidase from Streptococcus mutans is an exo-hydrolase that acts on the nonreducing terminal α-1,6-glucosidic linkage of oligosaccharides and dextran with a high degree of transglucosylation. Based on amino-acid sequence similarity, this enzyme is classified into glycoside hydrolase family 13. Recombinant dextran glucosidase was purified and crystallized by the hanging-drop vapour-diffusion technique using polyethylene glycol 6000 as a precipitant. The crystals belong to the orthorhombic space group P212121, with unit-cell parameters a = 72.72, b = 86.47, c = 104.30 Å. A native data set was collected to 2.2 Å resolution from a single crystal.

• International Union of Crystallographyの論文

著者

• 森 春英

  北海道大学大学院農学研究科応用生命科学専攻

• 森 春英

  北海道大学大学院農学研究院応用生命科学部門

• 木村 淳夫

  北海道大学大学院農学研究院応用生命科学部門

• Matsui Hirokazu

  Dept. Biosci. Chem. Fac. Agric. Hokkaido Univ.

• Matsui Hirokazu

  Hokkaido Univ. Sapporo Jpn

• Kobayasi Kazuhiro

  Department Of Agriculture Faculty Of Life And Environmental Science Shimane University

• Kimura Atsuo

  Hokkaido Univ.

• Kimura Atsuo

  Graduate School Of Agriculture Hokkaido University

• Okuyama Masayuki

  Research Faculty Of Agriculture Hokkaido University

• Kimura Atsuo

  Hokkaido University

• Matsui Hirokazu

  Laboratory Of Biochemistry Research Faculty Of Agriculture Hokkaido University

• HONDOH Hironori

  Research Faculty of Agriculture, Hokkaido University

• SABURI Wataru

  Research Faculty of Agriculture, Hokkaido University

• Hondoh Hironori

  Research Faculty Of Agriculture Hokkaido University

• Mori Haruhide

  Research Faculty Of Agriculture Hokkaido University

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