Calcium ion-dependent increase in thermostability of dextran glucosidase from Streptococcus mutans.
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概要
- 論文の詳細を見る
Dextran glucosidase from Streptococcus mutans (SmDG), which belongs to glycoside hydrolase family 13 (GH13), hydrolyzes the non-reducing terminal glucosidic linkage of isomaltooligosaccharides and dextran. Thermal deactivation of SmDG did not follow the single exponential decay but rather the two-step irreversible deactivation model, which involves an active intermediate having 39% specific activity. The presence of a low concentration of CaCl2 increased the thermostability of SmDG, mainly due to a marked reduction in the rate constant of deactivation of the intermediate. The addition of MgCl2 also enhanced thermostability, while KCl and NaCl were not effective. Therefore, divalent cations, particularly Ca2+, were considered to stabilize SmDG. On the other hand, CaCl2 had no significant effect on catalytic reaction. The enhanced stability by Ca2+ was probably related to calcium binding in the β→α loop 1 of the (β/α)(8) barrel of SmDG. Because similar structures and sequences are widespread in GH13, these GH13 enzymes might have been stabilized by calcium ions.
- 日本農芸化学会の論文
- 2011-08-23
著者
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森 春英
北海道大学大学院農学研究科応用生命科学専攻
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森 春英
北海道大学大学院農学研究院応用生命科学部門
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木村 淳夫
北海道大学大学院農学研究院応用生命科学部門
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Mori Haruhide
Research Faculty of Agriculture, Hokkaido University
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KIMURA Atsuo
Research Faculty of Agriculture, Hokkaido University
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Matsui Hirokazu
Dept. Biosci. Chem. Fac. Agric. Hokkaido Univ.
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Matsui Hirokazu
Hokkaido Univ. Sapporo Jpn
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Kobayasi Kazuhiro
Department Of Agriculture Faculty Of Life And Environmental Science Shimane University
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Kimura Atsuo
Hokkaido Univ.
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Kimura Atsuo
Graduate School Of Agriculture Hokkaido University
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Okuyama Masayuki
Research Faculty Of Agriculture Hokkaido University
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Kimura Atsuo
Hokkaido University
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Matsui Hirokazu
Laboratory Of Biochemistry Research Faculty Of Agriculture Hokkaido University
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KOBAYASHI Momoko
Research Faculty of Agriculture, Hokkaido University
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HONDOH Hironori
Research Faculty of Agriculture, Hokkaido University
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SABURI Wataru
Research Faculty of Agriculture, Hokkaido University
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Hondoh Hironori
Research Faculty Of Agriculture Hokkaido University
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Kobayashi Momoko
Research Faculty Of Agriculture Hokkaido University
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Mori Haruhide
Research Faculty Of Agriculture Hokkaido University
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Saburi Wataru
Research Faculty Of Agriculture Hokkaido University
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