Crystallization and preliminary crystallographic analysis of dextranase from Streptococcus mutans
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概要
- 論文の詳細を見る
Streptococcus mutans dextranase hydrolyzes the internal α-1,6-linkages of dextran and belongs to glycoside hydrolase family 66. An N- and C-terminal deletion mutant of S. mutans dextranase was crystallized by the sitting-drop vapour-diffusion method. The crystals diffracted to a resolution of 1.6 Å and belonged to space group P2_[1], with unit-cell parameters a = 53.2, b = 89.7, c = 63.3 Å, β = 102.3°. Assuming that the asymmetric unit of the crystal contained one molecule, the Matthews coefficient was calculated to be 4.07 Å^[3] Da^[-1]; assuming the presence of two molecules in the asymmetric unit it was calculated to be 2.03 Å 3 Da^[-1].
著者
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森 春英
北海道大学大学院農学研究科応用生命科学専攻
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森 春英
北海道大学大学院農学研究院応用生命科学部門
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Kim Young-min
Graduate School Of Agriculture Hokkaido University
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木村 淳夫
北海道大学大学院農学研究院応用生命科学部門
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Matsui Hirokazu
Dept. Biosci. Chem. Fac. Agric. Hokkaido Univ.
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Matsui Hirokazu
Hokkaido Univ. Sapporo Jpn
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Kobayasi Kazuhiro
Department Of Agriculture Faculty Of Life And Environmental Science Shimane University
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Kimura Atsuo
Hokkaido Univ.
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Kimura Atsuo
Graduate School Of Agriculture Hokkaido University
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Kim Young-min
Hokkaido Univ.
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Kim Young-min
Hokkaido University
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Kim Young-min
Special Business Division Koreaabrasive Ind. Co. Ltd.
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Okuyama Masayuki
Research Faculty Of Agriculture Hokkaido University
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Kimura Atsuo
Hokkaido University
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Funane K
National Food Research Institute
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Matsui Hirokazu
Laboratory Of Biochemistry Research Faculty Of Agriculture Hokkaido University
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Mori Haruhide
Research Faculty Of Agriculture Hokkaido University
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