Cloning and Heterologous Expression of a Glucodextranase Gene from Arthrobacter globiformis I42, and Experimental Evidence for the Catalytic Diad of the Recombinant Enzyme(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
スポンサーリンク
概要
- 論文の詳細を見る
The gene encoding a glucodextranase from Arthrobacter globiformis I42 was cloned and, subsequently, heterologously expressed in Escherichia coli. This glucodextranase gene consists of 1048 amino acid residues with a calculated molecular mass of 109, 135 Da. The roles of two residues at the active site of A. globiformis I42 glucodextranase were examined by site-directed mutagenesis. Glutamic acid residues 458 and 656, which are part of the apparent catalytic residues, were found to be essential for hydrolase activity.
- 社団法人日本生物工学会の論文
- 2004-02-25
著者
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Ito Hiroyuki
Department Of Internal Medicine Division Of Cardiovascular And Respiratory Medicine Akita University
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Matsui Hirokazu
Department of Bioscience and Chemistry, Faculty of Agriculture, Hokkaido University
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森 春英
北海道大学大学院農学研究院応用生命科学部門
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WATANABE Kenji
Department of Kampo Medicine, Keio University, School of Medicine
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Watanabe K
Faculty Of Pharmaceutical Science School Of Medicine Fukuoka University
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Matsui H
Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
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MORIMOTO NAOKI
Department of Applied Bioscience, Graduate School of Agriculture, Hokkaido University
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YASUKAWA YOSHITAKA
Department of Applied Bioscience, Graduate School of Agriculture, Hokkaido University
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UNNO TAKEHIRO
Nihon Shokuhin Kako Co., Ltd.
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Ito Hiroyuki
Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
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Matsui Hirokazu
Dept. Biosci. Chem. Fac. Agric. Hokkaido Univ.
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Matsui Hirokazu
Department Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
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Matsui Hirokazu
Hokkaido Univ. Sapporo Jpn
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Watanabe Katsunori
Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University
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Watanabe K
Tokyo Institute Of Technology Faculty Of Bioscience And Biotechnology Department Of Biological Scien
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Watanabe K
Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
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Unno Takehiro
Nihon Shokuhin Kako Co. Ltd.
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Morimoto Naoki
Department Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
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Watanabe K
Research Institute For Food Science Kyoto University:(present Address)department Of Agricultural Che
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Watanabe K
Department Of Applied Biological Sciences Faculty Of Agriculture Saga University
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Watanabe K
Marine Biotechnol. Inst. Iwate Jpn
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Ito H
Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
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Ito Hiroyuki
Department Of Cardiology Akita University
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Watanabe K
Department Of Chemistry And Life Science College Of Bioresource Sciences Nihon University
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Matsui Hirokazu
Laboratory Of Biochemistry Research Faculty Of Agriculture Hokkaido University
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Watanabe Kenji
Department Of Gastroenterology Osaka City University Graduate School Of Medicine
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Yasukawa Yoshitaka
Department Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
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Watanabe Kenji
Department Of Anatomy Fukui Medical School
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Ito Hiroyuki
Department Of Anesthesiology Tokyo Medical And Dental University Graduate School Of Medical And Dent
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Watanabe Keiichi
The Graduate School Of Science And Technology Kobe University
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Watanabe Kenji
Department Of Agricultural Chemistry College Of Agriculture And Veterinary Medicine Nihon University
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MATSUI Hirokazu
Department of Agricultural Chemistry, Faculty of Agriculture, Hokkaido University
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