Purification and Properties of Acid Phosphatase Secreted from Lupin Roots under Phosphorus-Deficiency Conditions
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概要
- 論文の詳細を見る
When lupin plants were grown in phosphorus-deficient nutrient solutions, the activity of acid phosphatase secreted from their roots remarkably increased in comparison with that under adequate phosphorus conditions. Based on activity staining of acid phosphatase on IEF gels, many isozymes were present in roots and leaves, but one of these was mainly secreted into the rhizosphere. This main acid phosphatase secreted from roots was purified from the phosphorus-deficient nutrient solution where lupin was grown, using ion exchange chromatography with DEAE-Sepharose CL-6B, gel filtration with Bio-Gel P-200, and preparative-PAGE. The molecular weight of the purified enzyme was estimated to be 72, 000 by SDS-PAGE and approximately 140, 000 by gel filtration. Thus, this enzyme was considered to be a homo-dimer. The isoelectric point and the optimum pH of the enzyme were 4.7 and 4.3, respectively.
- 社団法人日本土壌肥料学会の論文
著者
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Osaki Mitsuru
Faculty of Agriculture, Hokkaido University
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Matsui Hirokazu
Faculty of Agriculture, Hokkaido University
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Tadano Toshiaki
Faculty of Agriculture, Hokkaido University
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Matsui Hirokazu
Department of Bioscience and Chemistry, Faculty of Agriculture, Hokkaido University
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Ozawa Kenji
Department Of Bioscience And Chemistry Faculty Of Agriculture Hokkaido University
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Osaki Mitsuru
Faculty Of Agriculture Hokkaido University
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Matsui H
Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
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HONMA Mamoru
Faculty of Agriculture, Hokkaido University
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Tadano Toshiaki
Faculty Of Agriculture Hokkaido University
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Honma M
Department Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
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Honma Mamoru
Faculty Of Agriculture Hokkaido University
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Ozawa Kenji
Faculty Of Engineering Yamanashi University
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MATSUURA Hideyuki
Research Faculty of Agriculture, Hokkaido University
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Matsui Hirokazu
Laboratory Of Biochemistry Research Faculty Of Agriculture Hokkaido University
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Matsui Hirokazu
Lab. Of Biochemistry Div. Of Applied Bioscience Res. Fac. For Agriculture Hokkaido Univ.
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