Isolation and Characterization of a cDNA from Catharanthus roseus Which Is Highly Homologous with Phosphate Transporter
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概要
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The PIT1 gene which is highly homologous with phosphate transporter was isolated from Catharanthus roseus and analyzed. The cDNA PIT1 contained an open reading frame of 542 amino acids and its sequence showed a 31, 30, and 34% identity with the phosphate transporter of Saccharomyces cerevisiae (PHO84), Neurospora crassa (PHO-5), and Glomus versiforme (GvPT), respectively. Furthermore, the cDNA PIT1 encoded a highly hydrophobic protein with 12 putative membrane-spanning regions and contained a conserved amino acid sequence reported in the human glucose transporter superfamily. S. cerevisiae strain DpU (pho84 knockout strain) was unable to grow on low phosphate (55 μM) medium (LP medium). Expression of the PIT1 cDNA enabled DpU to grow on LP medium. Northern hybridization analysis revealed that the PIT1 gene was expressed in roots, stems, and young whole plant of C. roseus, but not in leaves.
- 社団法人日本土壌肥料学会の論文
著者
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Osaki Mitsuru
Faculty of Agriculture, Hokkaido University
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Tadano Toshiaki
Faculty of Agriculture, Hokkaido University
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Osaki Mitsuru
Faculty Of Agriculture Hokkaido University
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KIKUCHI Yasuhiro
Tsukuba Research Laboratories, Kyowa Hakko Co., Ltd.
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Tadano Toshiaki
Faculty Of Agriculture Hokkaido University
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Kai Motoshi
Criminal Science Laboratory, Hokkaido Police Headquarters
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Masuda Yukiko
Tsukuba Research Laboratory, Kyowa Hakko Kogyo Co. Ltd.
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Kikuchi Yasuhiro
Tsukuba Research Laboratories Kyowa Hakko Co. Ltd.:(present Address)tokyo Research Laboratories Kyow
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Kai Motoshi
Criminal Science Laboratory Hokkaido Police Headquarters
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Masuda Yukiko
Tsukuba Research Laboratory Kyowa Hakko Kogyo Co. Ltd.
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Kikuchi Yasuhiro
Tsukuba Research Laboratory Kyowa Hakko Kogyo Co. Ltd.
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