Biochemical Characterization of a Thermophilic Cellobiose 2-Epimerase from a Thermohalophilic Bacterium, Rhodothermus marinus JCM9785
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概要
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Cellobiose 2-epimerase (CE) reversibly converts glucose residue to mannose residue at the reducing end of β-1,4-linked oligosaccharides. It efficiently produces epilactose carrying prebiotic properties from lactose, but the utilization of known CEs is limited due to thermolability. We focused on thermoholophilic Rhodothermus marinus JCM9785 as a CE producer, since a CE-like gene was found in the genome of R. marinus DSM4252. CE activity was detected in the cell extract of R. marinus JCM9785. The deduced amino acid sequence of the CE gene from R. marinus JCM9785 (RmCE) was 94.2% identical to that from R. marinus DSM4252. The N-terminal amino acid sequence and tryptic peptide masses of the native enzyme matched those of RmCE. The recombinant RmCE was most active at 80 °C at pH 6.3, and stable in a range of pH 3.2–10.8 and below 80 °C. In contrast to other CEs, RmCE demonstrated higher preference for lactose over cellobiose.
- 2011-11-23
著者
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Matsui Hirokazu
Department of Bioscience and Chemistry, Faculty of Agriculture, Hokkaido University
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Matsui H
Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
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Mori Haruhide
Research Faculty of Agriculture, Hokkaido University
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Matsui Hirokazu
Dept. Biosci. Chem. Fac. Agric. Hokkaido Univ.
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Matsui Hirokazu
Hokkaido Univ. Sapporo Jpn
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MATSUURA Hideyuki
Research Faculty of Agriculture, Hokkaido University
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Saburi Wataru
Nihon Shokuhin Kako Co. Ltd.
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Matsui Hirokazu
Laboratory Of Biochemistry Research Faculty Of Agriculture Hokkaido University
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YAMAMOTO Takeshi
Nihon Shokuhin Kako Co., Ltd.
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Yamamoto Takeshi
Nihon Shokuhin Kako Co. Ltd.
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Matsui Hirokazu
Lab. Of Biochemistry Div. Of Applied Bioscience Res. Fac. For Agriculture Hokkaido Univ.
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OJIMA Teruyo
Nihon Shokuhin Kako Co., Ltd.
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SATO Hiroki
Research Faculty of Agriculture, Hokkaido University
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MATSUI Hirokazu
Research Faculty of Agriculture, Hokkaido University
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Mori Haruhide
Research Faculty Of Agriculture Hokkaido University
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