1-Aminocyclopropane-1-carboxylate (ACC) Deaminase Induced by ACC Synthesized and Accumulated in Penicillium citrinum Intracellular Spaces
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概要
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We have already described how 1-aminocy-clopropane-1-carboxylic acid (ACC), which is a precursor of the plant hormone ethylene, is synthesized in Penicillium citrinum through the same reaction by the catalysis of ACC synthase [EC 4.4.1.14] as in higher plants. In addition, ACC deaminase [EC 4.1.99.4], which degrades ACC to 2-oxobutyrate and ammonia, was also purified from this strain. To study control of induction of ACC deaminase in this organism, we have isolated and analyzed the cDNA of P. citrinum ACC deaminase and studied the expression of ACC deaminase mRNA in P. citrinum cells. By the analysis of peptides from the digests of the purified and modified ACC deaminase with lysylendopeptidase, 70% of its amino acid sequences were obtained. These amino acid sequences were used to identify a cDNA, consisting of 1,233 bp with an open reading frame of 1,080 bp encoding ACC deaminase with 360 amino acids. The deduced amino acids from the cDNA are identical by 52% and 45% to those of enzymes of Pseudomonas sp. ACP and Hansenula saturnus. Through Northern blot analysis, we found that the mRNA of ACC deaminase was expressed in P. citrinum cells grown in a medium containing 0.05% L-methionine. These findings suggest that ACC synthesized by ACC synthase and accumulated in P. citrinum intracellular spaces can induce the ACC deaminase that degrades the ACC.
- 社団法人日本農芸化学会の論文
- 2000-02-23
著者
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MATSUI Hirokazu
Graduate School of Agriculture, Hokkaido University
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Ito Hiroyuki
Graduate School of Agriculture, Hokkaido University
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Matsui H
Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
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HONMA Mamoru
Graduate School of Agriculture, Hokkaido University
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JIA YanJun
Graduate School of Agriculture, Hokkaido University
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Ito Hiroyuki
Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
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Matsui Hirokazu
Dept. Biosci. Chem. Fac. Agric. Hokkaido Univ.
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Matsui Hirokazu
Hokkaido Univ. Sapporo Jpn
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Jia Yanjun
Graduate School Of Agriculture Hokkaido University
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Honma M
Department Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
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Ito H
Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
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Ito Hiroyuki
Graduate School Of Agriculture Hokkaido University
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Jia Yan-Jun
Graduate School of Agriculture, Hokkaido University
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