Purification and Properties of Extracellular Carboxyl Proteases of Acid-tolerant Bacteria, Isolated from Compost
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概要
- 論文の詳細を見る
Four strains of acid-tolerant and protein-using bacteria were isolated from compost. Two of them, Gram-negative strains MB8 and MB11,were identified as a new genus close to Stenotrophomonas species MB8 and Burkholderia species MB11,respectively. Both bacteria produced extracellular carboxyl proteases (CP) in acid-casein-starch medium. The enzymes, termed CP MB8 and CP MB11,purified through ion exchange and gel filtration chromatographies had molecular weights of 61,000 (CP MB8) and 36,000 (CP MB11) as estimated bv SDS-PAGE, and showed optimum activities with hemoglobin as a substrate at pH 3.5 (CP MB8) and pH 3.7 (CP MB11) at 55℃. Both of the enzymes were not inhibited by pepstatin, DAN, or EPNP. These results suggest that both enzymes are members of the pepstatin-insensitive carboxyl proteins family (EC 3.4.23.33), except for a larger molecular weight of the CP MB8 enzyme.
- 社団法人日本農芸化学会の論文
- 1999-11-23
著者
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Ito Hiroyuki
Division of cardiology, Saitama Medical Center
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MATSUI Hirokazu
Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University
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Matsui H
Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
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TAKEHANA Toshihiko
Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University
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INOUE Sakura
Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University
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TAKEI Ryo
Tsukuba Chemical R&D Laboratory, Adeka Clean Aid Co., Ltd.
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HONMA Mamoru
Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University
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Ito Hiroyuki
Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
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Matsui Hirokazu
Dept. Biosci. Chem. Fac. Agric. Hokkaido Univ.
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Takei Ryo
Tsukuba Chemical R&d Laboratory Adeka Clean Aid Co. Ltd.
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Inoue Sakura
Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
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Honma M
Department Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
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Ito H
Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
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Matsui Hirokazu
Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
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Takehana Toshihiko
Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
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