Potent Inhibition of Macrophomate Synthase by Reaction Intermediate Analogs

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概要

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• Potent inhibitors for macrophomate synthase, which has recently been found to catalyze a highly unusual five-step chemical transformation, were explored. Among 11 oxalacetate analogs tested, only three analogs had moderate to relatively strong inhibitory activities (I_<50> 1.3-8.1mM). On the other hand, among 35 bicyclic intermediate analogs synthesized, two diacids were found to be the most potent inhibitors (I_<50> 0.80,0.84mM) which had a much higher affinity than that of the natural substrate 2-pyrone. (-)-Enantiomers of the diacids showed 30 times stronger activity (I_<50> 0.34,0.41mM) than (+)-ones. The I_<50>/K_m values (0.20,0.24) showed their potent inhibitions. Competitive inhibitions were observed in two representative inhibitors.

• 社団法人日本農芸化学会の論文
• 2000-11-23

著者

• Yagi K

  Department Of Applied Bioscience Graduate School Of Agriculture Hokkaido University

• Yagi Kensuke

  Faculty Of Agriculture Shizuoka University

• Watanabe K

  Faculty Of Pharmaceutical Science School Of Medicine Fukuoka University

• MIE Takashi

  Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University

• WATANABE Kenji

  Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University

• OIKAWA Hideaki

  Division of Chemistry, Graduate School of Science, Hokkaido University

• HONMA Mamoru

  Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University

• ICHIHARA Akitami

  Department of Bioscience and Chemistry, Faculty of Agriculture, Hokkaido University

• Ohashi S

  Laboratory Of Functional Anatomy Department Of Life Sciences Faculty Of Agriculture Meiji University

• Ohashi S

  Hokkaido Univ. Hokkaido Jpn

• Ohashi Satoshi

  Second Institute Of New Drug Discovery Otsuka Pharmaceutical Co. Ltd.

• YAGI Kenji

  Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University

• OHASHI Satoshi

  Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University

• ICHIHARA Akitami

  Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University

• KOBAYASHI Kimiko

  Molecular Characterization Division, RIKEN (The Institute of Physical and Chemical Research)

• Watanabe Katsunori

  Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University

• Watanabe K

  Tokyo Institute Of Technology Faculty Of Bioscience And Biotechnology Department Of Biological Scien

• Watanabe K

  Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University

• Ohashi Satoshi

  Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University

• Ichihara A

  Hokkaido Univ. Sapporo Jpn

• Mie Takashi

  Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University

• Honma M

  Department Of Applied Bioscience Graduate School Of Agriculture Hokkaido University

• Oikawa Hideaki

  Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University

• Watanabe K

  Research Institute For Food Science Kyoto University:(present Address)department Of Agricultural Che

• Watanabe K

  Department Of Applied Biological Sciences Faculty Of Agriculture Saga University

• Watanabe K

  Marine Biotechnol. Inst. Iwate Jpn

• Kobayashi Kimiko

  Molecular Characterization Division Riken (the Institute Of Physical And Chemical Research)

• Watanabe K

  Department Of Chemistry And Life Science College Of Bioresource Sciences Nihon University

• Watanabe Keiichi

  The Graduate School Of Science And Technology Kobe University

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