Substrate Specificity of β-Primeverosidase, A Key Enzyme in Aroma Formation during Oolong Tea and Black Tea Manufacturing(Food & Nutrition Science)
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概要
- 論文の詳細を見る
We synthesized nine kinds of diglycosides and a monoglycoside of 2-phenylethanol to investigate the substrate specificity of the purified β-primeverosidase from fresh leaves of a tea cultivar (Camellia sinensis var. sinensis cv. Yabukita) in comparison with the apparent substrate specificity of the crude enzyme extract from tea leaves. The crude enzyme extract mainly showed β-primeverosidase activity, although monoglycosidases activity was present to some extent.The purified β-primeverosidase showed very narrow substrate specificity with respect to the glycon moiety, and especially prominent specificity for the β-primeverosyl (6-0-β-D-xylopyranosyl-β-D-glucopyranosyl) moiety. The enzymes hydrolyzed naturally occurring diglycosides such as β-primeveroside, β-vicianoside, β-acuminoside, β-gentiobioside and 6-O-α-L-arabinofuranosyl-β-D-glucopyranoside, but were unable to hydrolyze synthetic unnatural diglycosides. The purified enzyme was inactive toward 2-phenylethyl β-D-glucopyranoside. The enzyme hydrolyzed each of the diglycosides into the corresponding disaccharide and 2-phenylethanol. These results indicate the β-primeverosidase, a diglycosidase, to be a key enzyme involved in aroma formation during the tea manufacturing process.
- 2001-12-23
著者
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平竹 潤
京大・化研
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WATANABE Naoharu
Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University
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Watanabe N
Faculty Of Agriculture Shizuoka University
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Watanabe Naoharu
Department Of Agricultural Chemistry The University Of Tokyo
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HAYASHI Kentaro
Department of Neurosurgery, Nagasaki University School of Medicine
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Kobayashi H
National Food Res. Inst. Ibaraki Jpn
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Higuchi Kouji
Department Of Biochemical Science And Technology Faculty Of Agriculture Kagoshima University
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Mizutani Masaharu
Institute for Chemical Research, Kyoto University
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Sakata Kanzo
Institute for Chemical Research, Kyoto University
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Yagi K
Department Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
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Hiratake Jun
Institute For Chemical Research Kyoto University
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Yagi Kensuke
Faculty Of Agriculture Shizuoka University
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Hayashi Kentaro
Department Of Neurosurgery Nagasaki University School Of Medicine
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YAGI Kensuke
Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University
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Sugiyama K
Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuoka University
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Mizutani M
Institute For Chemical Research Kyoto University
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Mizutani Masaharu
Institute For Chemical Research Kyoto University
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Hayashi K
Department Of Biochemical Sciences Kagoshima University
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MA Seung-Jin
Institute for Chemical Research, Kyoto University
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Ma Seung-jin
Institute For Chemical Research Kyoto University
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Sakata Kanzo
Institute For Chemical Research Kyoto University
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Sakata Kanzo
Faculty Of Agriculture Shizuoka University
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Hayashi Kunioki
Dep. Of Biochemical Sci. And Technol. Fac. Of Agriculture Kagoshima Univ. Jpn
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Sakata K
Institute For Chemical Research Kyoto University
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Hayashi Kentaro
Department Of Aeronautics And Astronautics Graduate School Of Engineering Kyushu University
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Hayashi Kentaro
Department Mechanical Engineering For Computer-controlled Machinery Osaka University
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