ジベレリン生合成にかかわるチトクロムP450cDNAの単離と機能解析

概要

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Gibberellines (GAs) are required for internode elongation and seed germination and also for complete anther, seed and fruit development. GA biosynthetic pathway has three different classes of enzymes, and each enzyme is located either in plastids, endoplasmic reticulum or cytoplasm. Most of genes encoding GA biosynthesis enzymes in plastids and cytoplasm have been isolated. On the other hand, cytochrome P450 genes encoding GA biosynthesis enzymes in the endoplasmic reticulum have not been isolated, except GA3. The objectives of our study are identification of cytochrome P450 enzymes which catalyze two steps of the reactions from ent-kaurenoic acid to GA_<12>-aldehyde,and clarification of regulation mechanismsof GA biosyntheses on the molecular basis. The P450 cDNAs belonging to CYP88 and CYP707 families have been isolated from Arabidopsis and tomato. Ent-Kaurenoic acid was synthesized from stevioside in order to identify a P450 which catalyze 7α-hydroxylation of ent-kaurenoic acid to form ent-7α-hydroxykaurenoic acid. The isolated P450 cDNAs were expressed in heterologous cells. Now, the measurement of ent-kurenoic acid 7α-hydroxylation activity is in progress.
植物化学調節学会の論文
2000-11-02