53 トリアゾール環を有するブラシノステロイド生合成阻害剤の構造活性相関と作用部位

概要

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Brz was selected through the screening for brassinosteroid biosynthesis inhibitors. In dark grown Arabidopsis, Brz-induced morphological changes were nearly restored to those of wild type by the additional treatment of brassinolide. The structure of Brz is similar to pacrobutrazol, a gibberellin biosynthesis inhibitor, the assays on cress plants, Brz-treated plants do not show recovery by the addition of gibberellin, but show good recovery by the addition of brassinolide. Brz-treated cress also showed dwarfism with altered leaf morphology, including the downward curling and dark-green color typical of Arabidopsis brassinosteroid deficient mutants, and this dwrfism was reversed by the application of 10 nM brassinolide. This result suggests that brassinosteroids are essential for plant growth and Brz can be used to clarify the function of brassinosteroids in plants as a complement to brassinosteroid-deficient mutants. The Brz action site was also investigated by feeding brassinosteroid biosynthesis intermediates to cress grown in the light. The results suggested that Brz blocks at least one step upstream of teasterone formation.
植物化学調節学会の論文
1999-10-01